IMAGING OXYGEN METABOLISM IN BRAIN

大脑中氧代谢成像

• 批准号: 6056640
• 负责人: UMAMAHESWAR DUVVURI
• 金额: $ 3.69万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6056640
• 关键词: bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain metabolism; cerebral ischemia /hypoxia; disease /disorder model; immunocytochemistry; laboratory rat; magnetic resonance imaging; model design /development; oxygen consumption; oxygen transport; stroke

DESCRIPTION (Adapted from applicant's abstract): Ischemic stroke is a common and devastating disorder. The long-term goal of this project is to develop a tool to study the pathophysiology of stroke and in particular the ischemic penumbra. In the ischemic core blood flow is <20 percent of normal and metabolism is almost absent. The peripheral regions also have reduced blood flow (20 to 50 percent of normal) but are only slightly hypometabolic. This region is called the "ischemic penumbra". It has been shown that this region can be salvaged with early treatment. These areas are also involved in active protein synthesis which is thought to endow ischemic tolerance. The applicant hypothesizes that in vivo magnetic resonance imaging (MRI) of oxygen consumption can distinguish penumbral regions from infarcted regions in the setting of focal ischemia. In order to test this hypothesis the applicant proposes to develop novel MR methods to image cerebral oxygen metabolism and blood flow. The methods are based on indirect detection of the 17-0 nucleus using 1H MRI, which has already been demonstrated to be a sensitive technique. The applicant will evaluate this method in normal rats and validate it against existing methods for measuring cerebral oxygen metabolism. He will then use this method to study a reversible model of stroke involving occlusion of middle cerebral artery. The phenomenon of ischemic tolerance will be investigated by correlating MR data with immunohistochemical assays for protein expression. In this way, he hopes to be able to correlate MR images with protein synthesis. This project will greatly enhance our understanding of the pathophysiology of stroke and possibly provide a tool with which to noninvasively assess genetic therapies.

描述（改编自申请人的摘要）：缺血性中风是一种 常见且具有破坏性的疾病。 该项目的长期目标是 开发一种工具来研究中风的病理生理学，特别是 缺血半暗带。 缺血性核心血流量<正常值的 20% 并且新陈代谢几乎不存在。 周边地区也有所减少 血流量减少（正常值的 20% 至 50%），但代谢程度仅轻微降低。 该区域被称为“缺血半影”。 事实证明，这 通过早期治疗可以挽救该区域。 这些领域也涉及 参与活性蛋白质合成，被认为具有缺血耐受性。 申请人假设体内磁共振成像（MRI） 耗氧量可以区分半暗区和梗死区 在局灶性缺血的情况下。 为了检验这个假设 申请人提议开发新的磁共振方法来成像脑氧 新陈代谢和血液流动。 该方法基于间接检测 使用 1H MRI 检测 17-0 核，该核已被证明是 敏感的技术。 申请人将在正常大鼠中评估该方法 并根据现有的脑氧测量方法进行验证 代谢。 然后他将使用这种方法来研究可逆模型 涉及大脑中动脉闭塞的中风。 现象 将通过将 MR 数据与 蛋白质表达的免疫组织化学测定。 通过这种方式，他希望 能够将 MR 图像与蛋白质合成相关联。 该项目将 极大地增强了我们对中风病理生理学的理解 可能提供一种非侵入性评估遗传的工具 疗法。