IMAGING OXYGEN METABOLISM IN BRAIN

大脑中氧代谢成像

• 批准号: 6056640
• 负责人: UMAMAHESWAR DUVVURI
• 金额: $ 3.69万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6056640
• 关键词: bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain metabolism; cerebral ischemia /hypoxia; disease /disorder model; immunocytochemistry; laboratory rat; magnetic resonance imaging; model design /development; oxygen consumption; oxygen transport; stroke

DESCRIPTION (Adapted from applicant's abstract): Ischemic stroke is a common and devastating disorder. The long-term goal of this project is to develop a tool to study the pathophysiology of stroke and in particular the ischemic penumbra. In the ischemic core blood flow is <20 percent of normal and metabolism is almost absent. The peripheral regions also have reduced blood flow (20 to 50 percent of normal) but are only slightly hypometabolic. This region is called the "ischemic penumbra". It has been shown that this region can be salvaged with early treatment. These areas are also involved in active protein synthesis which is thought to endow ischemic tolerance. The applicant hypothesizes that in vivo magnetic resonance imaging (MRI) of oxygen consumption can distinguish penumbral regions from infarcted regions in the setting of focal ischemia. In order to test this hypothesis the applicant proposes to develop novel MR methods to image cerebral oxygen metabolism and blood flow. The methods are based on indirect detection of the 17-0 nucleus using 1H MRI, which has already been demonstrated to be a sensitive technique. The applicant will evaluate this method in normal rats and validate it against existing methods for measuring cerebral oxygen metabolism. He will then use this method to study a reversible model of stroke involving occlusion of middle cerebral artery. The phenomenon of ischemic tolerance will be investigated by correlating MR data with immunohistochemical assays for protein expression. In this way, he hopes to be able to correlate MR images with protein synthesis. This project will greatly enhance our understanding of the pathophysiology of stroke and possibly provide a tool with which to noninvasively assess genetic therapies.

描述（改编自申请人的摘要）：缺血性中风是一个 常见和毁灭性的疾病。 该项目的长期目标是 开发一种工具来研究中风的病理生理学，尤其是 缺血性半月。 在缺血性核心流动中，血流<占正常的20％ 新陈代谢几乎不存在。 外围区域也减少了 血流（占正常的20％至50％），但仅略有下代谢。 该区域称为“缺血性半衰期”。 已经证明了这一点 可以通过早期治疗来挽救区域。 这些区域也涉及 在活性蛋白质合成中，被认为是缺血性耐受性的。 申请人假设体内磁共振成像（MRI） 氧气消耗可以区分半身区域和梗塞区域 在局部缺血的情况下。 为了检验这一假设 申请人建议开发新的MR方法来对脑氧化图像 代谢和血流。 这些方法基于间接检测 使用1H MRI的17-0核，已经证明是一个 敏感技术。 申请人将在正常大鼠中评估这种方法 并根据现有的测量脑氧的方法进行验证 代谢。 然后，他将使用这种方法研究可逆模型 中风涉及中大脑中动脉的阻塞。 现象的现象 缺血性耐受性将通过将MR数据与 用于蛋白质表达的免疫组织化学测定。 这样，他希望 能够将MR图像与蛋白质合成相关联。 这个项目将 极大地增强了我们对中风的病理生理学的理解和 可能提供一种可以非侵入性评估遗传的工具 疗法。