MODELING OF CELLULAR STRUCTURES AND MICROMOTION

细胞结构和微运动建模

• 批准号: 6202299
• 负责人: RICHARD SKALAK
• 金额: $ 15.83万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2001-06-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6202299
• 关键词: biomechanics; cell migration; cell nucleus; cytoplasm; cytoskeleton; erythrocytes; fluid flow; leukocytes; mathematical model; membrane model; microcirculation; microfilaments; molecular biology; nonblood rheology; physical model; tropomyosin; vascular endothelium

The general aim of this project is to develop quantitative, analytic and numerical models of cellular structures, active and passive cell deformations, and certain fluid flows which are of interest to the program project as a whole. The specific aims of the project are to model the velocity and stress distributions for separated flows in experimental flow channels, to model the rolling and detachment of adhering leukocytes in such flows, and to develop several structural models of leukocyte cytoskeleton, active motion, and stress transfer through endothelial cells. The biological and medical significance of the proposed research lies in the interaction with the other parts of the program project involving experimental studies. This project is intended to elucidate interpretations of experimental data and to test hypotheses concerning experimental results observed in other projects of the program project. The methods to be used are mathematical analysis, finite element methods, and computer graphics presentation. The proposed work will develop new computational methods for macroscopic structural models of intracellular cytoskeleton. The structural models will include adhesion molecules and their connection through the cell membrane to the cytoskeleton. The macroscopic results of cell deformation, rolling, adhesion, and physical properties will be subject to verification by comparison to experimental data generated by the other projects of the program project.

该项目的总体目的是开发定量，分析和 细胞结构，活性和被动细胞的数值模型 该程序感兴趣的变形和某些流体流动 整个项目。 该项目的具体目的是建模 实验流中分离流的速度和应力分布 通道，建模粘附白细胞的滚动和脱离 这样的流，并开发了白细胞的几种结构模型 细胞骨架，主动运动和应力通过内皮转移 细胞。 拟议研究的生物学和医学意义 在于与程序项目的其他部分的互动 涉及实验研究。 该项目旨在阐明 实验数据的解释并检验有关的假设 在计划项目的其他项目中观察到的实验结果。 要使用的方法是数学分析，有限元方法， 和计算机图形演示。 拟议的工作将开发新的 细胞内宏观结构模型的计算方法 细胞骨架。 结构模型将包括粘附分子和 它们通过细胞膜连接到细胞骨架。 这 细胞变形，滚动，粘附和物理的宏观结果 与实验相比，属性将经过验证 该计划项目的其他项目生成的数据。