GENETIC METHOD TO ANALYZE BACS AND CONSTRUCT CONTIG MAPS

分析 BACS 和构建重叠群图谱的遗传方法

• 批准号: 2828661
• 负责人: Alan Greener
• 金额: $ 9.96万
• 依托单位: STRATAGENE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2828661
• 关键词: Escherichia coli; analytical method; artificial chromosomes; gene targeting; genetic library; genetic mapping; genetic techniques; method development; molecular cloning; nucleic acid sequence; plasmids; transposon /insertion element

The goal of this proposal is to develop a genetic method to analyze BAC DNA clones and create a contig map of a BAC library. This method avoids the requirement of redundant sequencing in analyzing a BAC clone and provides a method for contig construction that does not involve hybridization or multiplex PCR analysis. PROPOSED COMMERCIAL APPLICATIONS: The system of transposons, plasmids, and E. coli host strains used would provide the research lab with the tools necessary to study individual sections of BAC clones and construct contig maps that do not require expensive instrumentation or large quantities of biochemical reagents.

该提案的目标是开发一种遗传方法来分析 BAC DNA 克隆并创建 BAC 文库的重叠群图谱。该方法避免了分析 BAC 克隆时的冗余测序要求，并提供了一种不涉及杂交或多重 PCR 分析的重叠群构建方法。拟议的商业应用：所使用的转座子、质粒和大肠杆菌宿主菌株系统将为研究实验室提供研究 BAC 克隆的各个部分和构建重叠群图谱所需的工具，而无需昂贵的仪器或大量生化试剂。