A9: MODALITIES TO CONTROL HIV 1 GENE EXPRESSION THROUGH RNA VIRAL VECTORS

A9：通过 RNA 病毒载体控制 HIV 1 基因表达的方式

• 批准号: 6205928
• 负责人: Ramasamy Raju
• 金额: $ 7.57万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205928
• 关键词: AIDS; biotechnology; genetics; genome; human tissue; immunology; infection; inflammation; lymphatic system; minority institution research support; virus

The human immunodeficiency virus (HIV-1) is a complex retrovirus with regard to its genome architecture and gene expression. The human retroviruses including HIV-1 code for specific viral proteins which regulate gene expression at the transcriptional and post-transcriptional levels. The transactivator of HIV transcription (Tat) is one such protein, which has been the subject of intense investigation. The first objective of this pilot project s the development and application lf RNA viral vectors to study the molecular mechanism of Tat protein function. RNA viral vectors represent a new generation of biomolecules which are being exploited to deliver specific RNA viral molecules into cells. The second objective of this pilot project involves the construction of RNA vectors carrying TAT specific antisense and decoy RNA sequences of HIV-1. The constructs will be used to inhibit the HIV-1 gene expression in tissue culture systems. These two objectives are interlinked, and involve very similar experimental strategies.

人类免疫缺陷病毒（HIV-1）是一种复杂的逆转录病毒 关于其基因组结构和基因表达。 人类 逆转录病毒包括 HIV-1 编码的特定病毒蛋白 在转录和调控基因表达 转录后水平。 HIV转录反式激活因子 (Tat) 就是这样一种蛋白质，它一直是人们强烈关注的主题。 调查。 该试点项目的第一个目标是 lf RNA病毒载体的开发和应用研究 Tat 蛋白功能的分子机制。 RNA病毒载体 代表正在开发的新一代生物分子 将特定的RNA病毒分子递送到细胞中。 第二个 该试点项目的目标是建设 RNA 携带 TAT 特异性反义和诱饵 RNA 序列的载体 HIV-1。 该构建体将用于抑制 HIV-1 基因 在组织培养系统中的表达。 这两个目标是 相互关联，并且涉及非常相似的实验策略。