CORE--BIOCHEMISTRY

核心--生物化学

• 批准号: 5215230
• 负责人: ROBERT H ROTH
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5215230
• 关键词: Cercopithecidae; Parkinson's disease; biochemistry; biomedical facility; dopamine; dopamine receptor; dopamine transporter; embryo /fetus cell /tissue; high performance liquid chromatography; homovanillate; nervous system transplantation

Although some promising data have been obtained in studies attempting to ameliorate experimental or clinical Parkinsonism, the technique of neural transplantation is still in its infancy. Controversy surrounds many issues in the field. This Core Unit is well situated to tackle some of these important questions. Biochemical and pharmacological techniques, including HPLC, GC-MS, autoradiography, and radioimmunoassay will be used to study; the factors that may influence the survival of fetal dopamine cells destined for transplantation, the functioning and growth of graft fibers following transplantation, the integration of the grafted cells with the host brain, the mechanism underlying the behavioral effects of transplantation, and ways to improve both the model of Parkinson's disease and the cells that are implanted. In addition, new approaches and techniques will be implemented as they become available. The Core pioneered the potential of novel cocaine analogs as highly sensitive markers for dopamine neurons. Quantitative autoradiography using two such analogs has yielded new insights into graft function in the striatum of the MPTP-treated monkey and contributed a useful ligand for imaging studies also now in progress.

尽管在研究中获得了一些有希望的数据 改善实验或临床帕金森氏症，神经技术 移植仍处于起步阶段。 争议围绕着许多问题 在现场。 这个核心单元的位置非常适合解决其中一些 重要问题。 生化和药理学技术，包括HPLC，GC-MS， 放射自显影和放射免疫测定法将用于研究； 因素 这可能会影响注定的胎儿多巴胺细胞的生存 移植，移植纤维的功能和生长 移植，将移植细胞与宿主大脑的整合， 移植行为影响的基础机制， 改善帕金森氏病和细胞的模型的方法 被植入。 此外，新的方法和技术将在它们的情况下实施 可用。 核心开创了新型可卡因的潜力 类似物作为多巴胺神经元的高度敏感标记。 定量 使用两个这样的类似物的放射自显影产生了对移植的新见解 在MPTP处理的猴子的纹状体中的功能，并贡献A 现在也在进行成像研究的有用配体。