INFLUENCE OF EMF ON FREE RADICAL MEDIATED TOXOCOLOGICAL PROCESSES

EMF 对自由基介导的毒理学过程的影响

• 批准号: 5202221
• 负责人: C F CHIGNELL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5202221
• 关键词: cytotoxicity; electromagnetic radiation; erythrocytes; free radicals; hemolysis; photochemistry; radiation carcinogenesis; toxicology; triplet state; ultraviolet radiation

Although epidemiological studies suggest that there is a weak relationship between exposure to electromagnetic fields (EMF) and cancer there are at present few plausible molecular mechanisms to explain this effect. The known involvement of free radicals in the etiology of cancer and other diseases raises the possibility that EMF may somehow act by increasing the lifetime and/or concentration of free radicals. It has been known for many years that in organized media (e.g. detergent micelles) applied magnetic fields cause an increase in average free radical concentration and lengthen the lifetime of free radicals. If these effects occur in vivo then this may explain some of the reported biological effects of EMF. Our initial studies have focused on the intact erythrocyte as a test system that could be used to study the effects of MF on free radical processes. We have found that ketoprofen, a phototoxic, non-steroidal anti-inflammatory drug (structurally related to benzophenone), caused complete hemolysis of human erythrocytes after only 20 min UV irradiation. When UV irradiation was carried out in a magnetic field (3500G) the time taken for hemolysis to occur in 50% of the cells (t) was significantly shortened (78 min vs. 96 min). This observation can be attributed to a magnetic field induced decrease in the rate of intersystem crossing of the geminate triplet radical pair generated by the reduction of ketoprofen in its triplet excited state by erythrocyte membrane components, probably lipids. To our knowledge this is the first demonstration of an effect of MF on a toxicological process that is known to occur via a free radical mechanism.

尽管流行病学研究表明 接触电磁场（EMF）与癌症之间的关系 目前，很少有合理的分子机制可以解释这一点 影响。 自由基参与癌症病因的已知参与 其他疾病提高了EMF可能以某种方式采取行动的可能性 增加自由基的寿命和/或浓度。 它有 多年来一直在有组织的媒体中闻名（例如洗涤剂 胶束）施加的磁场导致平均自由度增加 自由基浓度并延长自由基的寿命。 如果 这些影响发生在体内，这可能解释了一些报道的 EMF的生物学作用。 我们的最初研究集中于 完整的红细胞作为一种测试系统，可用于研究 MF对自由基过程的影响。我们发现酮洛芬， 一种非毒性，非甾体类抗炎药（结构相关的药物 到苯甲酮），引起人类红细胞的完全溶血 仅20分钟的紫外线照射。当紫外线照射在 磁场（3500G）溶血所花费的时间发生在50％ 细胞（T）显着缩短（78分钟与96分钟）。 这 观察可以归因于磁场引起的下降 甲壳虫三胞胎激进对的跨系统交叉速率 通过减少酮酮在其三重激发态下的酮产生 红细胞膜成分，可能是脂质。据我们所知 是MF对毒理学过程的第一次证明 已知这是通过自由基机制发生的。