DOPAMINE TRANSPORTER-- CELLULAR AND SUBCELLULAR LOCALIZATIONS

多巴胺转运蛋白——细胞和亚细胞定位

• 批准号: 5201668
• 负责人: G R UHL
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201668
• 关键词: basal lamina; brain cell; brain mapping; cocaine; dopamine transporter; drug receptors; electron microscopy; frontal lobe /cortex; immunocytochemistry; laboratory rat; light microscopy; reinforcer

The dopamine transporter (DAT) has been identified as the principal brain receptor site best correlated with the rewarding and euphoric properties of cocaine. Cellular and subcellular localization data are important to help to elucidate the functional properties of this important brain molecule. In previous FYs, investigators in this Branch described the detailed distributions of the dopamine transporter mRNA in cells of the rat and human brains. In this FY, studies of the distribution of the DAT protein in rat brain were substantially advanced. Light-microscopy revealed details of the distribution of DAT distribution in rat brain that included remarkably dense fiber distributions in specific laminae of the frontal cerebral cortex. Ultrastructural studies of the transporter revealed that most of the DAT immunoreactivity was found in intracellular and plasma membrane localizations not readily associated with conventional synaptic specializations. These studies substantially add to information on the detailed cellular localizations of these important sites for cocaine reward, and underscore the possibility that extrasynaptic removal of dopamine provides a principal function of the DAT.

多巴胺转运蛋白（DAT）已被确定为主要的大脑 与奖励和欣快特性最相关的受体位点 可卡因。 细胞和亚细胞定位数据对于 有助于阐明这个重要大脑的功能特性 分子。 在之前的财政年度中，该部门的调查人员描述了 多巴胺转运蛋白 mRNA 在细胞中的详细分布 老鼠和人类的大脑。 在本财年，对 DAT 分布的研究 大鼠大脑中的蛋白质含量显着提高。 光学显微镜 揭示了大鼠大脑中 DAT 分布的细节 其中包括特定薄片中非常密集的纤维分布 额叶大脑皮层。 超微结构研究 转运蛋白显示大部分 DAT 免疫反应性存在于 细胞内和质膜定位不易关联 具有传统的突触专门化。 这些研究实质上 添加有关这些详细细胞定位的信息 可卡因奖励的重要场所，并强调以下可能性： 突触外多巴胺的去除提供了 数据。