ROLE OF OLIGOSACCHARIDES IN THE EXPRESSION & FUNCTION OF IMMUNE RECEPTORS

低聚糖在表达中的作用

• 批准号: 5201055
• 负责人: K KEARSE
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201055
• 关键词: B lymphocyte; MHC class I antigen; MHC class II antigen; T cell receptor; T lymphocyte; endoplasmic reticulum; glycoprotein biosynthesis; molecular chaperones; oligosaccharides; protein folding; protein structure function; receptor expression

Upon insertion into the lumen of the endoplasmic reticulum (ER), many newly synthesized proteins are modified by addition of oligosaccharide side chains to asparagine residues (N-linked sugar chains). Processing of N-linked sugar chains is initiated within the ER by sequential removal of glucose residues by glucosidase I and glucosidase II enzymes, a process referred to as glucose trimming. A growing body of evidence signifies that glucose trimming is important for association of many nascent glycoproteins with calnexin, a molecular chaperon that functions in the assembly/folding of oligomeric protein complexes. Indeed, calnexin association is believed to be an important step in the assembly of several major immune receptor proteins, including major histocompatibility complex (MHC) class I and class II molecules and the antigen receptors expressed on T and B lymphocytes. We studied the role of glucose trimming and calnexin association in the assembly of MHC class I proteins and T cell antigen receptor (TCR) proteins.

插入内质网 (ER) 腔后，许多 新合成的蛋白质通过添加寡糖进行修饰 天冬酰胺残基的侧链（N-连接糖链）。 N-连接糖链的加工是在 ER 内启动的 通过葡萄糖苷酶 I 连续去除葡萄糖残基和 葡萄糖苷酶 II 酶，这一过程称为葡萄糖修剪。 一个 越来越多的证据表明血糖调整很重要 对于许多新生糖蛋白与钙联蛋白的关联， 分子伴侣在组装/折叠中发挥作用 寡聚蛋白质复合物。 事实上，钙联接蛋白协会是 相信这是几个主要国家集结的重要一步 免疫受体蛋白，包括主要组织相容性复合物 (MHC) I 类和 II 类分子和抗原受体 表达于T和B淋巴细胞。 我们研究了葡萄糖的作用 MHC I 类组装中的修剪和钙连接蛋白关联 蛋白质和 T 细胞抗原受体 (TCR) 蛋白质。