A MFBS SCOR AT THE MOUNT DESERT ISLAND BIOLOGICAL LAB

沙漠岛山生物实验室的 MFBS SCOR

• 批准号: 3105957
• 负责人: DAVID H EVANS
• 金额: $ 29.28万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-27 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3105957
• 关键词: fresh water environment; heavy metals; ion transport; membrane permeability; metal metabolism; metal poisoning; saltwater environment

A Marine and Freshwater Biomedical Sciences Specialized Center of Research is proposed which will utilize components of the administration, facilities, and scientific staff of the Mt. Desert Island Biological Laboratory. The MFBS SCOR will provide the administrative and technical assistance, experimental animals, supplies, and capital equipment to promote the interaction, collaboration, and training of biomedical scientists (both clinicians and physiologists) investigating the toxic effects of heavy metals on membrane function. In addition, it will provide for the rapid dissemination of data derived from these studies. Animal systems will be used which model both passive and active membrane transport processes found in the human renal, intestinal and hepatic epithelia--tissues with known sensitivity to heavy metal toxicity. Specifically, research groups will examine the effects of heavy metals on: 1. Transport of ammonia, H, and HCO3 across the fish gill; 2. Transport of K by the fish urinary bladder; 3. Transport of glucose and amino acids across flounder renal microvesicles, and cotransport of NaCl/KC1 across shark rectal gland microvesicles; 4. Transport of NaCl/KC1 across isolated rectal gland tubules; 5. Maintenance of intracellular pH, Ca concentration, and membrane PD in isolated rectal gland cells; 6. Cellular work of isolated, perfused, and in vitro rectal gland; 7. Transport of amino acids across the fish gut; 8. Volume regulation by rectal gland cells; 9. Stimulus/response coupling of hormonal stimulation of transport of Cl, and vasoactivity, of fish gills; 10. Transport pathways for Na, K and Cl across the fish urinary bladder; 11. Glomerular permeability of the hagfish kidney; 12. Transport of Na and Cl across the fish gill; 13. Transport of Na and Cl across the fish cornea; as well as, 14. The mode of transport of methlymercury across the hepatic duct epithelium. It is proposed that this MFBS SCOR will provide a unique milieu for research and training of present and future biomedical scientists interested in the cellular and sub-cellular mechanisms of the toxic effects of heavy metals.

海洋和淡水生物医学科学研究中心 提议将利用行政部门的组成部分， 荒山岛生物研究所的设施和科研人员 实验室。 MFBS SCOR 将提供行政和技术支持 援助、实验动物、用品和资本设备 促进生物医学领域的互动、协作和培训 科学家（临床医生和生理学家）调查有毒物质 重金属对细胞膜功能的影响。 此外，还将提供 以便快速传播这些研究得出的数据。 动物 将使用模拟被动和主动膜运输的系统 人类肾脏、肠道和肝脏中发现的过程 上皮细胞——已知对重金属毒性敏感的组织。 具体来说，研究小组将研究重金属对以下方面的影响： 1.氨、H和HCO3穿过鱼鳃的运输； 2. 运输 K由鱼的膀胱； 3. 葡萄糖和氨基酸的运输 穿过鲽鱼肾微泡，以及 NaCl/KC1 的共转运 鲨鱼直肠腺微泡； 4. 跨隔离的 NaCl/KC1 运输 直肠腺管； 5.维持细胞内pH、Ca浓度、 和分离的直肠腺细胞中的膜PD； 6. 蜂窝工作 分离、灌注和体外直肠腺； 7. 氨基酸的运输 穿过鱼肠； 8.直肠腺细胞的体积调节； 9. Cl 运输的激素刺激的刺激/反应耦合，以及 鱼鳃的血管活性； 10. Na、K 和 Cl 的转运途径 鱼的膀胱； 11.盲鳗的肾小球通透性 肾; 12. Na和Cl穿过鱼鳃的运输； 13. 运输 Na 和 Cl 穿过鱼角膜；以及 14. 运输方式 甲基汞穿过肝管上皮。 建议 MFBS SCOR 将为 当前和未来生物医学科学家的研究和培训 对毒性作用的细胞和亚细胞机制感兴趣 的重金属。