EFFECTS OF ALCOHOL AND ACETATE ON LEUKOCYTE MEMBRANE RECEPTOR EXPRESSION

酒精和乙酸对白细胞膜受体表达的影响

• 批准号: 3808639
• 负责人: J M JONES
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3808639
• 关键词: CD antigens; MHC class I antigen; MHC class II antigen; acetates; alcoholic beverage consumption; alcoholism /alcohol abuse; antibody receptor; antigen receptors; flow cytometry; hemodialysis; human subject; leukocytes; monocyte; receptor expression; stainings; uremias

The purpose was to determine whether chronic exposure to moderate to high blood levels of acetate would result in alterations of expression of selected membrane markers. For this purpose, we examined alcoholic subjects exposed to acetate via alcohol metabolism (up to 10 fold physiological levels) and hemodialysis patients exposed via the dialysis buffers (up to 100 fold physiological levels). Acute exposure to high levels of alcohol was reported by others to enhance the expression of HLA class I antigens. In the present study, the expression of HLA class I (ABC), Class II (Dr) and CD16 Fc receptor antigens were measured by flow cytometry on blood leukocytes from alcoholic and hemodialyzed uremic patients. By staining with fluor=labeled monoclonal antibodies, we detected a small but not significant increase in expression (intensity) of HLA class I antigens on lymphocytes and monocytes of alcoholic subjects in comparison with a reference population of normal controls. Hemodialysis subjects exhibited a decreased expression of HLA class I. Alcoholic and hemodialysis subjects both showed a significant decrease in expression of HLA class II antigens. The average percentage of lymphocytes from alcoholics that expressed CD16 was similar to that of controls. Hemodialyzed subjects exhibited a decrease percent of lymphocytes that stained for CD16 but an increase percent of monocytes that stained for CD16. The results were compatible with a hypothesis that altered expression of HLA class I reported by others was an alcohol effect while the altered expression of class II and CD16 that we observed was an acetate effect.

目的是确定是否长期暴露于中度至高度 血液中乙酸盐的浓度会导致 选定的膜标记。为此，我们检查了酗酒者 通过酒精代谢暴露于醋酸盐（高达生理学的 10 倍） 水平）和血液透析患者通过透析缓冲液暴露（高达 100 倍生理水平）。急性暴露于高浓度酒精 据其他人报道可以增强 HLA I 类抗原的表达。 本研究中HLA I类(ABC)、II类(Dr)的表达 通过流式细胞术测量血液中的 CD16 Fc 受体抗原 来自酒精和血液透析尿毒症患者的白细胞。通过染色 使用荧光=标记的单克隆抗体，我们检测到了一个小的但不是 HLA I 类抗原的表达（强度）显着增加 与正常人相比，酗酒者的淋巴细胞和单核细胞 正常对照的参考人群。血液透析受试者表现出 HLA I 类表达减少。酒精和血液透析受试者 两者均显示 HLA II 类抗原的表达显着降低。 酗酒者中表达 CD16 的淋巴细胞的平均百分比 与对照相似。进行血液透析的受试者表现出下降 CD16 染色的淋巴细胞百分比，但增加的百分比 CD16 染色的单核细胞。结果与 其他人报告的 HLA I 类表达改变的假设是 酒精效应同时改变了 II 类和 CD16 的表达 观察到的是醋酸盐效应。