EFFECT OF DIET ON ALCOHOL (AND ALDEHYDE) DEHYDROGENASES

饮食对酒精（和醛）脱氢酶的影响

• 批准号: 3108895
• 负责人: WILLIAM F BOSRON
• 金额: $ 8.92万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-02-01 至 1987-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3108895
• 关键词: acetaldehyde; affinity chromatography; alcohol dehydrogenase; alcoholic beverage consumption; aldehyde dehydrogenases; dietary proteins; ethanol; fasting; gel electrophoresis; isozymes; nutrition related tag; radiotracer

The objective of this project is to evaluate the role of liver alcohol dehydrogenase and aldehyde dehydrogenase in the regulation of alcohol metabolism in various nutritional or physiologic states. We have shown that fasting of rats or hamsters for 48th results in a concominant decrease of both ethanol elimination rate in vivo and of the maximal alcohol dehydrogenase activity measured in vitro. Since NAD ion/NADH ratios were shown to be similar in fed and fasted animals following ethanol administration, the decrease in elimination rate cannot be explained by alterations in NAD ion or NADH levels. We have also shown that castration of mature rats results in a 70% increase in enzyme activity. This is consistent with the increase in alcohol metabolism observed previously for castrated animals. Since the alcohol metabolic rate appears to be regulated by the content of alcohol dehydrogenase in these physiologic states, we propose to isolate the enzyme forms under these conditions and examine their steady state kinetic properties and their synthesis and degradation rates. These studies should provide a biochemical basis of understanding for the regulation of alcohol metabolism in various nutritional and physiologic states.

该项目的目的是评估肝脏酒精的作用 脱氢酶和乙醛脱氢酶在酒精代谢调节中的作用 处于各种营养或生理状态。 我们已经证明禁食 大鼠或仓鼠第 48 次结果导致乙醇同时减少 体内消除率和最大乙醇脱氢酶活性 体外测量。 由于 NAD 离子/NADH 比率在饲料中显示相似 和禁食动物乙醇给药后，消除减少 速率不能用 NAD 离子或 NADH 水平的变化来解释。 我们还有 研究表明，成熟大鼠的去势导致酶增加 70% 活动。 这与观察到的酒精代谢增加一致 以前用于阉割的动物。 由于酒精代谢率似乎受酒精含量的调节 乙醇脱氢酶在这些生理状态下，我们建议分离 酶在这些条件下形成并检查其稳态动力学 性质及其合成和降解率。 这些研究应该 为理解酒精调节提供生化基础 各种营养和生理状态下的新陈代谢。

项目成果

Purification and characterization of mouse alcohol dehydrogenase from two inbred strains that differ in total liver enzyme activity.

总肝酶活性不同的两种近交系小鼠乙醇脱氢酶的纯化和表征。

• DOI: 10.1007/bf00499291
• 发表时间: 1984
• 期刊: Biochemical genetics
• 影响因子: 2.4
• 作者: Rex,DK;Bosron,WF;Li,TK
• 通讯作者: Li,TK

Kinetic characterization of two classes of dog liver alcohol dehydrogenase isoenzymes.

两类狗肝乙醇脱氢酶同工酶的动力学特征。

• DOI: 10.1111/j.1530-0277.1985.tb05740.x
• 发表时间: 1985
• 期刊: Alcoholism, clinical and experimental research
• 作者: Bosron,WF;Gaither,JW;Magnes,LJ
• 通讯作者: Magnes,LJ

Purification and characterization of the Danish (Skive) variant of mouse liver alcohol dehydrogenase.

小鼠肝脏乙醇脱氢酶丹麦 (Skive) 变体的纯化和表征。

• DOI: 10.1007/bf00498955
• 发表时间: 1987
• 期刊: Biochemical genetics
• 影响因子: 2.4
• 作者: Rex,DK;Bosron,WF;Dwulet,F;Li,TK
• 通讯作者: Li,TK