Single-cell gene profiling during early cardiomyogenesis in mice

小鼠早期心肌发生过程中的单细胞基因分析

• 批准号: G0900105/1
• 负责人: Kenta Yashiro
• 金额: $ 42.48万
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0900105%2F1
• 关键词: Single; cell; gene; profiling; during

Generation of heart cells in the laboratory is very useful in both scientific research and clinical therapy. These cells are not only a good alternative to animals which are currently used for heart research but also are a valuable material for treating patients with heart failure, which is a major killer to the UK people. However, in order to achieve this goal, it is essential to further understand how heart cells develop in our body before birth.This study aims to unveil characteristics (?gene profiles?) of specific cells (heart cell precursors) in the embryo which are changing to heart cells. This very important information has not been achieved due to technical limitations of previously-available methods because such cells are extremely small in number. This study will solve such difficulties by using a combination of state-of-the art technologies. In the experiment, a single individual cell of interest will be isolated and collected from embryo under microscope, and the genes included in the cell will be amplified using a newly-established molecular biological method so that the amount of the genes become sufficient for assessment. Then, amplified gene expression of each cell will be assessed by using the latest, extremely powerful analyser. The data obtained will provide a range of new important knowledge regarding how heart cells are developed in the body, which will in turn suggests how to produce heart cells from stem cells in the laboratory. Moreover, this information may open a door to new research. We may be able to generate heart cells from patient?s own cells such as skin cells by applying the information obtained in this study. Thus this research project will gratefully contribute to the progress in medical science and to development of new therapies for heart failure.

实验室中心脏细胞的产生在科学研究和临床疗法中非常有用。这些细胞不仅是目前用于心脏研究的动物的良好替代品，而且是治疗心力衰竭患者的宝贵材料，这对英国人群来说是一个主要的杀手。但是，为了实现这一目标，必须进一步了解出生前的心脏细胞在我们体内如何发展。这项研究旨在揭示特定细胞（心脏细胞前体）（心脏细胞前体）的特征（基因谱），这些特定细胞（心脏细胞前体）正在变成心脏细胞。由于以前可用的方法的技术局限性，由于这些细胞的数量极少，因此尚未实现此非常重要的信息。这项研究将通过结合最先进的技术来解决此类困难。在实验中，将在显微镜下从胚胎中分离并收集单个感兴趣的单个细胞，并且细胞中包含的基因将使用新建立的分子生物学方法进行扩增，以使基因的量足以评估。然后，将使用最新的，极强大的分析仪评估每个细胞的放大基因表达。获得的数据将提供有关体内心脏细胞如何发展的一系列新重要知识，这反过来又暗示了如何从实验室中的干细胞中产生心脏细胞。此外，此信息可能打开了新研究的大门。通过应用本研究中获得的信息，我们可能能够从患者自己的细胞（例如皮肤细胞）中产生心脏细胞。因此，该研究项目将对医学的进步和发展新疗法的发展深表感谢。