PURCHASE OF A CIRCULAR DICHROISM SPECTROPHOTOMETER

购买圆二色分光光度计

基本信息

批准号：
3519872
负责人：
JOHN H DAWSON
金额：
$ 11.1万
依托单位：
UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-04-18 至 1989-04-17
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3519872
关键词：
biomedical equipment purchase circular dichroism site directed mutagenesis spectrometry

项目摘要

Funds are requested for the purchase of a state of the art circular dichroism spectrophotometer with accessories for stopped flow and low temperature experiments and with a 15 kilogauss electromagnet to enable magnetic circular dichroism to be carried out. The new instrument will replace a thirteen year old model that has become obsolete and unreliable. The new instrument is twenty times more sensitive than the old model and is configured for simultaneous measurement of UV-visible absorption and circular dichroism spectra. The new instrument will also be equipped to make measurements from 175 to 1050 nm, a much expanded spectral window from that currently available. The specific areas of research of the primary users will focus on the use of the new instrument for the following purposes: (a) the continued development of magnetic circular dichroism spectroscopy as a probe of heme iron coordination structure and the initial examination of the use of this technique to study chlorin iron systems, (b) the application of circular dichroism spectroscopy to investigate and quantify the allosteric interconversion of the R and T states of chemically modified hemoglobins, (c) the use of stopped flow circular dichroism spectroscopy to study the mechanism of action of native thymidylate synthase and the use of circular dichroism spectroscopy to characterize selected mutant enzymes generated by site directed mutagenesis, and (d) the investigation of the circular dichroism properties of selenium containing biomolecules, particularly seleno-sulfides and di-selenides, in order to evaluate the potential utility of the method as a probe of the structure of selenobiomolecules.

需要资金购买最先进的通告带有停流附件的二色性分光光度计和低温实验以及15公斤电磁铁使磁性圆二色性执行。新乐器将取代已有十三年历史的乐器已经过时且不可靠的模型。新的仪器比旧型号灵敏二十倍，配置为同时测量紫外-可见光吸收光谱和圆二色光谱。新仪器还将配备进行 175 至 1050 nm 测量的设备，与当前可用的光谱窗口相比，有了更大的扩展。主要用户的具体研究领域将集中于使用新文书的目的如下： (a) 磁圆二色性的持续发展光谱作为血红素铁配位结构的探针使用该技术进行初步检查研究二氢卟酚铁系统，(b) 圆二色性的应用光谱法研究和量化变构化学修饰的R和T态的相互转化血红蛋白，(c) 使用停流圆二色性光谱学研究天然药物的作用机制胸苷酸合酶和圆二色性的应用光谱学来表征所产生的选定突变酶通过定点诱变，以及（d）对含硒生物分子的圆二色性，特别是硒硫化物和二硒化物，以便评估该方法作为结构探针的潜在效用硒生物分子。