FLUORESCENT ACTIVATED CELL SORTER UPGRADE SYSTEM

荧光激活细胞分选机升级系统

• 批准号: 3520070
• 负责人: DAVID A HORWITZ
• 金额: $ 12.9万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-05-16 至 1989-05-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520070
• 关键词: biomedical equipment purchase; biomedical equipment resource; cell sorting; clinical biomedical equipment; fluorescence

The purpose of this proposal is to update the service provided by the USC Flow Cytometry Core Facility. Since 1980 a Becton- Dickinson FACS IV has been available to members of the USC Cancer Center. This service has been used by 15 NIH supported members of the Cancer Center who are involved in basic and clinical investigations. The FACS is used for analysis and purification by cell sorting, DNA and/or RNA analysis, and various two color procedures including cell cycle analysis of specific lymphocyte subsets. We are requesting replacement of worn out equipment and new modifications which have become available since 1980. This equipment consists of new lasers, a computer, and a cooling system to preserve the life of expensive lasers. With this equipment we will improve our graphic presentation of the data and we will be able to perform three color analysis of surface markers, a procedure which will enable us to define more precisely functionally specific lymphocyte subsets, and to analyze more precisely the cell cycle of specific lymphocyte subsets. This new equipment will allow us the flexibility to develop new uses for the flow cytometer as dictated by our current and future users.

本提案的目的是更新由 南加州大学流式细胞术核心设施。 自 1980 年以来，Becton- Dickinson FACS IV 已向 USC 会员开放 癌症中心。 该服务已被 15 个 NIH 支持的机构使用 癌症中心的成员，他们参与基础和 临床研究。 FACS 用于分析和 通过细胞分选、DNA 和/或 RNA 分析进行纯化，以及 各种双色程序，包括细胞周期分析 特定的淋巴细胞亚群。 我们要求更换 破旧的设备和新的修改已成为 自 1980 年起推出。该设备由新型激光器、 计算机和冷却系统以维持昂贵的使用寿命 激光。 有了这个设备，我们将改善我们的图形 数据的呈现，我们将能够执行三个 表面标记的颜色分析，该程序将能够 我们更准确地定义功能特异性淋巴细胞 子集，并更精确地分析特定的细胞周期 淋巴细胞亚群。 这个新设备将使我们能够 根据规定灵活地开发流式细胞仪的新用途 由我们当前和未来的用户。