500 MHZ NMR INSTRUMENTATION FOR SHARED USE

供共享使用的 500 MHz NMR 仪器

基本信息

批准号：
3521167
负责人：
CRAIG ARTHUR TOWNSEND
金额：
$ 40万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-04-15 至 1992-10-14
项目状态：
已结题

项目摘要

A high field (500 MHz), multinuclear spectrometer is requested to meet the needs of a user group at the Homewood campus of The Johns Hopkins University from the Departments of Chemistry and Biology. A range of projects of chemical and biological significance is described: (1) Biosynthetic studies of aflatoxins and of Beta-lactam antibiotics, interactions of calicheamicin with oligonucleotides. (2) Synthetic and structural investigations of oligosaccarides, glycopeptides and glycolipids for research in carbohydrate recognition and hepatic receptor binding studies. (3) Structural studies of metal-binding peptides of the "zinc finger" type and related retroviral proteins. (4) Investigation of RNA folding and RNA-protein interactions. (5) Development of new synthetic methods and their application to total asymmetric natural product syntheses. (6) Chemical modeling of oxygen- binding and oxygen-activating copper protein active sites and investigations of the metal-binding properties of glutamyl-cysteinyl . peptides. (7) Structural studies of DNA molecules and oligonucleotides with adenine tracts to understand the origins of DNA bending. (8) Identification by NMR techniques of the labeled sites of alpha- chymotrypsin inhibited with 13C-enriched enzyme-activated inhibitors. Examination of chemi- and bioluminescent reaction mechanisms. Study by low-temperature NMR of deaminatively formed carbonium ions. (9) Oxygenation of hindered and deformed olefins and the reactions and rearrangements of carbenes will be investigated. (10) Synthesis of prostaglandins and their aza-analogues. (11) Studies of the mechanism of action and design of inhibitors for human placental aromatase and lanosterol 14alpha-demethylase.

高场（500 MHz），要求多核光谱仪满足约翰·霍普金斯（Homewood）校园的用户组需求大学化学与生物学系的大学。范围描述了化学和生物学意义的项目：（1）黄曲霉毒素和β-内酰胺抗生素的生物合成研究，卡利希氨基与寡核苷酸的相互作用。（2）合成和寡糖剂，糖肽和用于研究碳水化合物识别和肝的糖脂受体结合研究。（3）金属结合的结构研究 “锌指”类型和相关逆转录病毒蛋白的肽。（4）研究RNA折叠和RNA蛋白相互作用的研究。（5）开发新的合成方法及其在总计中的应用非对称天然产物合成。（6）氧的化学模型 - 结合和氧气激活铜蛋白活性位点和研究谷氨酰基 - 囊样金属结合特性。肽。（7）DNA分子和寡核苷酸的结构研究用腺嘌呤道理解DNA弯曲的起源。（8）通过NMR技术识别α-的标记位点用13C富集的酶激活抑制剂抑制胰凝乳蛋白酶。化学和生物发光反应机制的检查。研究脱氨基形成的碳离子的低温NMR。（9）阻碍和变形的烯烃的氧合以及反应以及将研究Carbenes的重排。（10）合成 Prostaglandins及其Aza-Analogues。（11）研究机制对人胎盘芳香酶和抑制剂的作用和设计羊轮14α-二甲基酶。