DIAGNOSIS OF INFLAMMATORY DIARRHEA

炎性腹泻的诊断

• 批准号: 3489570
• 负责人: ROBERT James CARMAN
• 金额: $ 3.83万
• 依托单位: TECHLAB, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3489570
• 关键词: antiserum; biomarker; diagnosis design /evaluation; diarrhea; enzyme linked immunosorbent assay; feces analysis; fluoresceins; fluorimetry; inflammation; lactoferrin; leukocytes; protease inhibitor; rapid diagnosis

Diarrhea, which is a major health problem throughout the world, is classified into either inflammatory or non-inflammatory, with inflammatory diarrheas being significantly more serious for the patient. The diagnosis which is critical in order to effectively treat the patient, is considerably more accurate and cost-effective if initial screening is done to determine if the diarrhea is inflammatory or non-inflammatory. The following project will evaluate assays designed to detect lactoferrin released from leukocytes in fecal specimens from patients with inflammatory diarrhea. We will optimize a rapid agglutination assay for the detection of lactoferrin using antibody-coated latex particles and examine the stability of the marker in fecal specimens. In addition, we will determine if dip-stick tests that detect lacoferrin are suitable. The project will be done using various standard and fecal specimens from patients with various types of diarrhea. The tests, which will be scaled up for commercial application, should be useful for the initial screening of diarrheal specimens and should make the diagnosis more efficient and less costly.

腹泻是全世界的一个主要健康问题 分为炎症性和非炎症性，其中炎症性 腹泻对患者来说明显更加严重。 诊断 这对于有效治疗患者至关重要 如果完成初步筛选，准确度和成本效益都会显着提高 以确定腹泻是炎症性还是非炎症性。 这 以下项目将评估旨在检测乳铁蛋白的测定 患者粪便标本中的白细胞释放 炎症性腹泻。 我们将优化快速凝集测定 使用抗体包被的乳胶颗粒检测乳铁蛋白和 检查粪便样本中标记物的稳定性。 此外，我们 将确定检测乳铁蛋白的试纸测试是否合适。 该项目将使用来自以下国家的各种标准和粪便样本来完成： 各种类型的腹泻患者。 测试将按比例进行 商业应用，应该对初步筛选有用 腹泻标本应该使诊断更加有效 成本较低。