TRANSFORMATION OF MAMMARY EPITHELIAL CELLS IN VITRO

乳腺上皮细胞的体外转化

• 批准号: 3446600
• 负责人: KEITH G DANIELSON
• 金额: $ 5.2万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446600
• 关键词: breast neoplasms; cancer registry /resource; cell bank /registry; cell differentiation; cellular oncology; chemical carcinogen; chemical carcinogenesis; mammary epithelium; neoplasm /cancer transplantation; preneoplastic state; tissue /cell culture; tumor promoters

The overall goal of the proposed project is to establish a transformation system in vitro for mammary epithelial cells that could be utilized for studies of chemical carcinogenesis at a cellular or molecular level. A new epithelial cell line, COMMA-ID, established by the principal investigator from normal mouse mammary tissue will be used in the proposed project to accomplish this goal. This cell line has advantages over previously characterized mammary cell lines in that the cells express diagnostic characteristics of normal mammary epithelial cells, (i.e. biosynthesis of casein polypeptides in vitro and ductal outgrowth in vivo that is morphologically indistinguishable from the normal mammary gland). These characteristics will permit use of this cell line for experiments relating to the following specific aims: 1. To correlate phenotypic properties in vitro of carcinogen-treated COMMA-ID cells with growth properties of the cells in vivo. 2. To establish and characterize preneoplastic and neoplastic cell lines from lesions derived from carcinogen-treated COMMA-ID cells transplanted in vivo. 3. To examine the conversion of preneoplatic cells to the tumorigenic phenotype by treatment of the cells in vitro with phorbol, a known promoting agent of mammary gland cancer in vivo. Correlations of the acquisition of transformation markers in vitro with growth potential in vivo will be made. 4. To investigate the relationship between expression of a differentiated phenotype (casein synthesis) and development of tumorigenicity. The proposed studies should provide insight into the important phenotypic alterations of mammary epithelial cells in vitro that are associated with preneoplastic and neoplastic growth in vivo.

拟议项目的总体目标是建立转型 乳腺上皮细胞体外系统，可用于 在细胞或分子水平上研究化学致癌作用。 一个新的 上皮细胞系，COMMA-ID，由首席研究员建立 来自正常小鼠乳腺组织的样本将在拟议的项目中使用 实现这个目标。 该细胞系比以前的细胞系具有优势 乳腺细胞系的特征在于细胞表达诊断 正常乳腺上皮细胞的特征（即生物合成 体外酪蛋白多肽和体内导管生长 形态上与正常乳腺没有区别）。 这些 特性将允许使用该细胞系进行相关实验 达到以下具体目标： 1. 关联表型特性 经致癌物处理的 COMMA-ID 细胞的体外培养，其生长特性 体内的细胞。 2. 建立并表征肿瘤前和 来自经致癌物处理的 COMMA-ID 病变的肿瘤细胞系 细胞移植体内。 3. 检查癌前细胞的转化 通过体外处理细胞，使细胞产生致瘤表型 佛波醇，一种已知的体内乳腺癌促进剂。 体外转化标记物获取与 体内就会产生生长潜力。 4. 调查关系 差异表型的表达（酪蛋白合成）和 致瘤性的发展。 拟议的研究应提供对重要表型的深入了解 体外乳腺上皮细胞的改变与 体内肿瘤前和肿瘤生长。