CORE--EXPOSURE AND ANALYTICAL FACILITY

核心——曝光和分析设施

• 批准号: 3777236
• 负责人: CARROLL A. SNYDER
• 金额: --
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3777236
• 关键词: Annelida; Malacostraca; X ray; aerosols; alternatives to animals in research; analytical chemistry; animal care; animal colony; atomic absorption spectrometry; bioassay; biomedical facility; cadmium; chromium; environmental toxicology; fluorescence; laboratory rat; mercury; method development; nickel; seafood poisoning; soil pollution; tissue /cell preparation; tissue resource /registry; toxicant screening; water pollution; water supply

The Exposure and Analytical Core functions as a resource for all investigators within this Program Project and acts as a conduit of services between the Biomedical and Ecological components. This Core provides services in three main areas: 1.) animal handling and exposure, this includes mammals, invertebrates and aquatic organisms; 2.) chemical analysis and sample preparation, including assays of toxicants in tissue, blood sediment, and water; 3.) animal cell and tissue preparation, including isolation of homogeneous cell populations, surgery and necropsy. This Core furnishes a unifying focus for biomarker development by providing cell and tissue preparations from animals exposed under identical conditions to the Biomedical components. This allows for direct comparisons of the effectiveness of the biomarker assays under development. In addition, this Core conducts investigatory projects designed to provide information concerning the bioavailability of toxicants to the Biomedical components. These investigatory projects are performed in collaboration with members of the Ecology components who provide expertise regarding routes of exposure, relevant dosages and pharmacokinetic analysis. Thus the Core uses data provided by the Ecology components to furnish bioavailability information to the Biomedical components. In this way the Core integrates the findings of the Ecology components into the studies conducted by the Biomedical components. In the past, the Core conducted a pharmacokinetic analysis of the clearance of chromium from various organs after chronic exposure to chromate. In the coming grant period, the Core will conduct two investigatory studies, one concerns the bioavailability of ingested cadmium bound to protein in food while the other concerns the bioavailability of chromium after pulmonary exposure to chromate- contaminated soil. Both of these studies will be conducted with investigators of the Ecology components, and the cell and tissue samples from exposed animals will be delivered to investigators in the Biomedical components. Thus, these activities of the Core will serve to integrate the expertise of the Ecology and Biomedical components and provide services to both.

曝光和分析核心作为所有人的资源 该计划项目中的调查人员并充当服务的渠道 在生物医学和生态成分之间。 此核心提供 在三个主要领域的服务：1。）动物处理和暴露，这 包括哺乳动物，无脊椎动物和水生生物； 2.）化学 分析和样品制备，包括在组织中毒性的测定 血液沉积物和水； 3.）动物细胞和组织制备， 包括分离均质细胞群，手术和死灵症。 该核心通过提供生物标志物开发的统一重点 来自相同暴露的动物的细胞和组织制剂 生物医学成分的条件。 这允许直接 比较正在开发的生物标志物测定的有效性。 此外，该核心进行了旨在提供的调查项目 有关毒物对生物医学的生物利用度的信息 成分。 这些调查项目是由合作执行的 与生态组成部分的成员提供有关专业知识的专业知识 暴露途径，相关剂量和药代动力学分析。 因此 该核心使用生态组件提供的数据来提供 生物医学组成部分的生物利用度信息。 这样 核心将生态成分的发现纳入研究 由生物医学成分进行。 过去，核心进行了 从各种器官清除铬的药代动力学分析 长期暴露于铬酸盐之后。 在未来的赠款期间，核心 将进行两项研究研究，其中一项涉及 摄取的镉与食物中的蛋白质结合，而另一个则关注 肺暴露于铬酸盐后铬的生物利用度 被污染的土壤。 这两项研究都将与 生态成分的研究者以及细胞和组织样品 从暴露的动物中将交付给生物医学的研究者 成分。 因此，核心的这些活动将有助于整合 生态和生物医学组成部分的专业知识，并为 两个都。