PERIPHERAL AND CENTRAL NERVOUS SYSTEM PEPTIDE NEUROTRANSMITTER RECEPTORS

周围和中枢神经系统肽神经递质受体

• 批准号: 3782467
• 负责人: E MEZEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3782467
• 关键词: biopsy; central nervous system; duodenum; gastric inhibitory peptide; gastrointestinal system; histamine receptor; human tissue; in situ hybridization; laboratory rat; muscarinic receptor; neurotransmitter receptor; peripheral nervous system; phagocytes; stomach

We have followed up on our findings that showed the presence of certain receptors in the immune cells of the lamina propria in the gut. We have extended the studies to biopsies from human stomach and duodenum and experimental conditions in the rat. We found that the presence of the receptors studied (Histamine2, gastrin and muscarinic receptors) is very similar between rats and human. We have studied several methodical problems and worked out modification of the in situ hybridization method so that it can be used in immune cells without the problems that made it difficult to evaluate results quanti~tatively. We found that there is an artificial amplification of the autoradiographic signal in cells with a large amount of oxidative enzymes (phagocytic cells) and that this can be prevented. We have studied the distribution of a recently cloned peptide receptor in the rat CNS (GIP or gastric inhibitory polypeptide receptor). We have found that the GIP receptor has a very unique localization suggesting that it may have a role in regulating blood pressure and limbic functions. We have also mapped its peripheral distribution, and found it present in many vascular endothel cells supporting its likely involvement in the regulation of blood flow~blood pressure.

我们跟进了我们的发现，这些发现表明存在某些 肠道中椎板的免疫细胞中的受体。我们有 将研究扩展到人类胃和十二指肠的活检和 大鼠的实验条件。 我们发现存在 研究的受体（组胺2，胃蛋白和毒蕈碱受体）非常 大鼠和人之间的相似之处。 我们研究了几条有条不紊的 问题并确定原位杂交方法的修改 因此，它可以在免疫细胞中使用，而不会出现问题 很难量化结果。我们发现有一个 用A的人工放大自显影信号 大量氧化酶（吞噬细胞），这可以 被阻止。 我们研究了最近克隆的肽受体的分布 在大鼠中枢神经系统（GIP或胃抑制多肽受体）中。我们有 发现GIP受体具有非常独特的本地化建议 它可能在调节血压和边缘 功能。我们还绘制了其外围分布，并发现了它 存在于许多支持其可能参与的血管内皮细胞中 在调节血流〜血压中。