IG GENETICS--ONTOGENY AND DIFFERENTIATION OF CELLS OF THE RABBIT IMMUNE SYSTEM

IG遗传学--兔免疫系统细胞的个体发育和分化

• 批准号: 3768720
• 负责人: R G MAGE
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3768720
• 关键词: B lymphocyte; cell differentiation; gene expression; gene rearrangement; gut associated lymphoid tissue; immunoglobulin genes; immunoglobulins; laboratory rabbit; lectin; leukopoiesis; nucleic acid sequence

We use techniques of immunogenetics and of molecular biology to study the rabbit immunoglobulins (Igs), and related genes such as the recombination activating genes RAG-1 and RAG-2 which are necessary for gene rearrangements to occur during lymphocyte development. We investigate the development of anatomical sites such as appendix follicles and germinal centers in gut associated lymphoid tissues and the regulated expression and sequence diversification of Ig genes during lymphoid cell development. Membrane IgD has not been definitively identified on rabbit B cells; the genetic region which should contain the delta gene has been cloned, but sequences corresponding to delta heavy chain exons have not been identified. We have identified two surface immunoglobulin complexes on rabbit peripheral blood B cells that possess heavy chains of similar apparent molecular size confirming previous reports of the presence of a second surface receptor expressed on rabbit B cells which may be IgD or an IgM-related molecule. In noncovalent association with one or both of these receptors are several proteins that are components of surface immunoglobulin (mIg) complexes expressed on rabbit B cells. Three proteins (42 kDa, 37kDa, 36kDa) were found in association with IgM expressed on B cells from peripheral blood and spleen. These proteins may be the rabbit homologues of murine Ig-beta (B29), Ig-gamma (truncated B29) and Ig-alpha (mb-1). Lectins are allowing us to dissect rabbit appendix follicles and germinal centers into subcompartments based on local expression of glycosylated cell surface receptors. We found two additional IgM-associated protein complexes containing 36kDa and 34kDa proteins in lysates from individual follicles isolated from the appendix. The appendix may be functioning early in development like the chicken bursa or sheep ileal Peyer's patch. However, the rabbit appendix does not involute but may alter its function and become a secondary lymphoid organ in the adult.

我们使用免疫遗传学和分子生物学技术来研究 兔免疫球蛋白（IGS）和相关基因，例如重组 激活基因rag-1和rag-2是基因所必需的 在淋巴细胞发育过程中发生重排。我们调查 诸如附录卵泡和生发等解剖位点的发展 肠道相关淋巴组织中的中心和调节表达 淋巴样细胞发育过程中Ig基因的序列多样化。 膜IgD尚未在兔B细胞上明确鉴定。这 应该包含三角基因的遗传区域已被克隆，但 与三角洲重链外显子相对应的序列尚未 确定。 我们已经确定了两个表面免疫球蛋白复合物 兔外周血B细胞具有相似的重链 明显的分子大小证实了先前存在的报道 在可能是IgD或A的兔B细胞上表达的第二表面受体 与IgM相关的分子。 在与一个或两个的非共价关联​​中 这些受体是几种是表面成分的蛋白质 在兔B细胞上表达的免疫球蛋白（MIG）复合物。 三 蛋白质（42 kDa，37KDA，36KDA）与IgM相关 用外周血和脾脏在B细胞上表达。 这些蛋白可能 成为鼠IG-beta（B29），IG-Gamma（截断B29）的兔同源物 和Ig-Alpha（MB-1）。 凝集素允许我们剖析兔附录 基于局部 糖基化细胞表面受体的表达。我们发现了两个 IgM相关的蛋白质复合物，含有36KDA和34KDA蛋白 从附录分离出的单个卵泡的裂解物。 这 附录可能在开发的早期运行，例如鸡bursa或 绵羊回肠佩耶的补丁。 但是，兔子附录不涉及 但是可能会改变其功能，并成为次级淋巴管的 成人。