BODIPY Photocage-Enabled Visualization and Optical Control of saRNA Delivery and Immunogenicity
BODIPY Photocage 实现 saRNA 传递和免疫原性的可视化和光学控制
基本信息
- 批准号:EP/X027252/2
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:英国
- 项目类别:Fellowship
- 财政年份:2024
- 资助国家:英国
- 起止时间:2024 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
RNA-based vaccines because of their enhanced immune response and cell-free manufacturing have become a potent and economical alternative to conventional vaccination approaches during the ongoing SARS-CoV-2 pandemic. The intrinsic single-stranded nature of RNA, however, oftentimes results in chemical instability and thus attenuated efficiency. One way to circumvent this issue is to use advanced delivery strategies which can stabilize RNA structures as well as allow efficient in vivo delivery and release. The objective of this proposal is to develop a light-responsive and fluorescent self-reporting RNA delivery system through integrating NIR light activated BODIPY photocages (Bpc) into RNA-loaded cationic polymers. In the current design, Bpc function both as the emissive self-reporters and photosensitive units. The Bpc emission will allow for real-time monitoring of the nanocarrier distribution and the photocaging process will enable on-demand release of the loaded RNA using light. To further explore this delivery platform, I will also investigate the delivery and controlled release properties of the nitro-functionalized Bpc (BpcNO2), the light-dependent fluorescence change of which can serve as an internal indicator to quantify the amount of released RNA. The success of the proposed work will offer 1) a novel, straightforward and biocompatible strategy to construct NIR light responsive and fluorescence traceable RNA delivery systems which are capable of maintaining the structural integrity and ensuring the translational efficiency of RNAs and 2) a chemical biology tool to probe the in vivo distribution and modulate the dose of immunogenic RNAs.
基于 RNA 的疫苗由于其增强的免疫反应和无细胞制造,在当前的 SARS-CoV-2 大流行期间已成为传统疫苗接种方法的有效且经济的替代方案。然而,RNA 固有的单链性质常常导致化学不稳定,从而降低效率。解决这个问题的一种方法是使用先进的递送策略,该策略可以稳定 RNA 结构并允许有效的体内递送和释放。该提案的目的是通过将近红外光激活的 BODIPY 光笼 (Bpc) 集成到负载 RNA 的阳离子聚合物中,开发一种光响应和荧光自报告 RNA 递送系统。在当前的设计中,Bpc 既充当发射自报告器又充当感光单元。 Bpc 发射将允许实时监测纳米载体的分布,并且光笼过程将能够利用光按需释放加载的 RNA。为了进一步探索这个递送平台,我还将研究硝基功能化 Bpc (BpcNO2) 的递送和控释特性,其光依赖性荧光变化可以作为量化释放 RNA 量的内部指示剂。这项工作的成功将提供:1)一种新颖、简单且生物相容的策略来构建近红外光响应和荧光可追踪的 RNA 传递系统,该系统能够保持结构完整性并确保 RNA 的翻译效率;2)一种化学生物学工具探测体内分布并调节免疫原性 RNA 的剂量。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Molly Stevens其他文献
Fabrication of calcium phosphate microcapsules using emulsion droplets stabilized with branched copolymers as templates.
使用支化共聚物稳定的乳液滴作为模板制造磷酸钙微胶囊。
- DOI:
10.1039/c5tb00893j - 发表时间:
2015-07-01 - 期刊:
- 影响因子:7
- 作者:
Robert V. Bell;Luke A. Rochford;Rafael T. M. de Rosales;Molly Stevens;Jonathan V. M. Weaver;Stefan A. F. Bon - 通讯作者:
Stefan A. F. Bon
Applications: general discussion
- DOI:
10.1039/c6fd90051h - 发表时间:
2016-09 - 期刊:
- 影响因子:3.4
- 作者:
Alberto Striolo;Francois Sicard;Luis Liz-Marzán;Catherine Murphy;Anna Roig;Axel Mueller;Javier Reguera;Yu Zhou;Mathias Brust;Leonardo Scarabelli;Luciano Tadiello;Antoine Thill;Irene Yarovsky;Martin Mayer;M. Arturo López-Quintela;Christian Kuttner;Estefania Gonzalez Solveyra;Heiko Wolf;Euan Kay;Lucia Pasquato;David Buceta;David Portehault;Hedi Mattoussi;Guillermo González;Roland Faller;David French;Benjamin Abécassis;Molly Stevens;Younan Xia;Richard Jones;Marcin Grzelczak;Matthew Penna;Calum Drummond - 通讯作者:
Calum Drummond
Opportunities
机会
- DOI:
10.1142/9789814566650_0014 - 发表时间:
1970-01-01 - 期刊:
- 影响因子:0
- 作者:
Sponsorship Brochure;Molly Stevens;Daniel Fletcher;Donny Liang - 通讯作者:
Donny Liang
Molly Stevens的其他文献
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{{ truncateString('Molly Stevens', 18)}}的其他基金
Development of a 3D-printed anisotropic heart-on-a-chip for drug screening applications
开发用于药物筛选应用的 3D 打印各向异性芯片心脏
- 批准号:
EP/X02721X/2 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Fellowship
Self-Illuminated PDT Platform for Highly Specific Diagnosis and Therapeutics for Deep Sited Tumor
用于深部肿瘤高度特异性诊断和治疗的自发光 PDT 平台
- 批准号:
EP/Y036646/1 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Fellowship
Unravelling coupling between multiscale tissue mechanics and heart valve calcification
揭示多尺度组织力学与心脏瓣膜钙化之间的耦合
- 批准号:
EP/X027163/2 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Fellowship
Novel minimally-invasive in-situ 3D bioprinting platform for cardiac regeneration
用于心脏再生的新型微创原位3D生物打印平台
- 批准号:
EP/X027287/2 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Fellowship
Say Yes to NO: The Next Generation Scaffolds with Localized and Sustained Nitric Oxide (NO) Delivery for Central Nervous System Regeneration
对“否”说“是”:具有局部和持续一氧化氮 (NO) 输送的下一代支架,用于中枢神经系统再生
- 批准号:
EP/X027198/2 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Fellowship
Bioinspired Multiplexed Ultrasensitive Biosensing
仿生多重超灵敏生物传感
- 批准号:
EP/Z000130/1 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Research Grant
Development of a 3D-printed anisotropic heart-on-a-chip for drug screening applications
开发用于药物筛选应用的 3D 打印各向异性芯片心脏
- 批准号:
EP/X02721X/1 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Fellowship
Unravelling coupling between multiscale tissue mechanics and heart valve calcification
揭示多尺度组织力学与心脏瓣膜钙化之间的耦合
- 批准号:
EP/X027163/1 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Fellowship
BODIPY Photocage-Enabled Visualization and Optical Control of saRNA Delivery and Immunogenicity
BODIPY Photocage 实现 saRNA 传递和免疫原性的可视化和光学控制
- 批准号:
EP/X027252/1 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Fellowship
相似海外基金
BODIPY Photocage-Enabled Visualization and Optical Control of saRNA Delivery and Immunogenicity
BODIPY Photocage 实现 saRNA 传递和免疫原性的可视化和光学控制
- 批准号:
EP/X027252/1 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Fellowship