HUMAN CELL CULTURE STUDIES OF MUTAGENS IN THE ABERJONA BASIN

阿伯霍纳盆地诱变剂的人类细胞培养研究

• 批准号: 3733677
• 负责人: WILLIAM G THILLY
• 金额: --
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3733677
• 关键词: B lymphocyte; arsenic; biomarker; chromium; denaturing gradient gel electrophoresis; environmental toxicology; family genetics; gel electrophoresis; gene mutation; human genetic material tag; human population genetics; human subject; human tissue; industrial waste; method development; mitochondrial DNA; mutagens; nucleic acid sequence; polymerase chain reaction; ribosomal RNA; tissue /cell culture; toxicant interaction; waste disposal; water pollution

Description: River and lake sediments from the Aberjona Valley have been shown to mutate human cells in short term assays. The chemical responsible for most of the mutagenicity of the organic sediment phase has been identified as benzo(a)pyrene. Its abundance correlates well with the local use of coal for home heating over the past century. The inorganic chemical mutagenicity of sediments of the Aberjona is however much greater than the organic group. Most of this activity may be ascribed to chromate deposited after two major releases circa 1930 and 1960 from the Aberjona headwater in and about the present Industriplex Superfund Site. Humans are usually exposed to water-borne chemicals at concentrations low enough to justify an assumption of independent action. This is claimed not to be the case for our estimate for chromate (4muM) and arsenite (1muM) in East Woburn drinking water drawn from municipal wells G and H. Thus, the intention is to examine the mutagenic results of arsenite interaction with chromate and other mutagens. Furthermore, human body water concentrations would be significantly lower than drinking water levels. They therefore consider it necessary to examine the mutagenicity of the sediments' chemicals at concentrations covering the range expected for children from birth to adolescence. In addition, they propose to extend these studies of stream and lake sediments into the two additional sub-basins which feed the Mystic Lakes proposed for study in the hydrogeology projects 1-6. The proposal includes obtaining mutational spectra from human cell cultures exposed to raw river sediments, chromate and chromate/arsenite mixtures. Long-term low dose protocols will be used to recreate the conditions calculated to have existed in children drinking the contaminated water from wells G and H. This project combined with the core laboratory and project 1 is responsible for identifying human mutagens in the Aberjona basin. Short term tests are carried out on sediments and the mutagens identified. As chromate and arsenite have been identified in well water, the influence of these and other mutagenic compounds are to be tested in human cell cultures. The wells providing drinking water for Woburn MA residents contain 4uM chromate and 1 uM in arsenite in the period 1964 - 1979. The specific aims of this project is to determine the biological meaning of exposure to such chemicals.

描述：阿伯乔纳山谷的河流和湖泊沉积物已经 显示在短期测定中突变人类细胞。化学物质 负责有机沉积物阶段的大部分诱变性 已被确定为苯并（a）pyrene。它的丰富性很好 在过去的一个世纪中，煤炭在本地使用煤炭进行供暖。这 然而 比有机组大得多。大部分活动可能是 归因于1930年大约两个主要版本后沉积的铬酸盐和 1960年，来自目前的Industriplex的Aberjona Headwater 超级基金网站。 人类通常以低浓度接触水传播化学物质 足以证明独立行动的假设。这就是声称 我们对铬酸盐（4MUM）和砷的估计并不是这样 （1MUM）在东沃本（East Woburn H.因此，目的是检查砷的诱变结果 与铬酸盐和其他诱变剂的相互作用。此外，人体 水浓度将大大低于饮用水 水平。因此，他们认为有必要检查 沉积物化学物质的诱变，浓度覆盖 从出生到青春期的儿童期望的范围。另外，他们 建议将这些溪流和湖泊沉积物的研究扩展到 另外两个供供研究的神秘湖泊的子巴斯金 在水文地质项目1-6中。 该提案包括从人类细胞中获得突变光谱 暴露于生河沉积物，铬酸盐和铬酸盐/砷的培养物 混合物。长期低剂量方案将用于重新创建 饮酒的儿童中计算出的条件已计算出来 来自Wells G和H的水受污染。 该项目与核心实验室和项目1相结合是 负责识别Aberjona盆地中的人类诱变剂。短的 对沉积物和诱变剂进行了术语测试。 由于在井水中已经确定了铬酸盐和砷， 这些和其他诱变化合物的影响应在 人类细胞培养。为沃本（Woburn Ma）提供饮用水的井 居民在1964年的砷中包含4UM铬酸盐和1 um -um- 1979年。该项目的具体目的是确定生物学 暴露于此类化学物质的含义。