XENOTRANSPLANTATION EVALUATION--HUMAN TISSUE/NUDE MICE

异种移植评估——人体组织/裸鼠

• 批准号: 3725516
• 负责人: JANET H RANSOM
• 金额: $ 32.97万
• 依托单位: BIOTECHNOLOGY RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-30 至 1997-06-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3725516
• 关键词: animal breeding; animal care; animal colony; athymic mouse; histopathology; human tissue; information systems; neoplasm /cancer transplantation; tissue /cell preparation; transplantation resource /registry /referral center; xenotransplantation

SUMMARY OF OBJECTIVES: To provide immunodeficient, a thymic nude mice, adequate facilities, and expertise for long-term studies of human tissue xenotransplants as the biological assay system for studying human carcinogenesis. GENERALLY: Provide a self-sustaining, nude mouse colony for Long-term studies of fresh human tissues, and/or in vitro induced transformation in human cells for in vivo expression and transplantation of malignancies in mice for cancer interruption studies. SPECIFICALLY: Provide as routine capability (1) 12 month survival studies of human tissues and cells transplanted subcutaneously as in vivo implants with viable epithelium and normal histology, in a self- sustaining, barrier colony accessible only by antibacterial personal showers and autoclaves for supplies; (2) Long-term survival studies of human organ tissue xenografts; (3) Long-term growth, proliferation, and tumorigenicity studies of cells for in vivo expression of preneoplastic and neoplastic transformation experimentally induced by selected chemical agents and genetic transfections. Materials Pick-up & delivery, Histopathology slide preparations and diagnoses, maintenance and expansion of the NCI-developed, dBASE-IBM PC colony data base, and veterinarian health care for breeders and experimental animals must support the continuing assay requirement of 10 scientists initiating an average of 50 new experiments, using 2,000+ mice/year.

目的摘要： 提供免疫缺陷、胸腺裸鼠、 足够的设施和专业知识来进行人体组织的长期研究 异种移植作为研究人类的生物测定系统 致癌作用。 一般：提供一个自我维持的裸鼠 用于新鲜人体组织和/或体外长期研究的菌落 在人体细胞中诱导转化以进行体内表达 小鼠恶性肿瘤移植用于癌症阻断研究。 具体来说： 提供常规能力 (1) 12 个月生存率 皮下移植人体组织和细胞的研究 具有存活上皮和正常组织学的体内植入物，以自我 维持屏障菌落，只有抗菌人员才能进入 供应品的淋浴和高压灭菌器； (2) 长期生存研究 人体器官组织异种移植； (3) 长期生长、增殖和 细胞体内表达的致瘤性研究 实验诱导的癌前和肿瘤转化 选定的化学试剂和基因转染。材料领取 和交付、组织病理学载玻片制备和诊断、 维护和扩展 NCI 开发的 dBASE-IBM PC 集群数据 饲养员和实验动物的基地和兽医保健 动物必须支持 10 的持续检测要求 科学家平均发起 50 项新实验，使用 2,000 多个 小鼠/年。