PROCUREMENT OF CELLS & DNA FOR GENETIC LINKAGE STUDIES

电池采购

• 批准号: 3626486
• 负责人: ROBERT J HAY
• 金额: $ 30.31万
• 依托单位: AMERICAN TYPE CULTURE COLLECTION
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3626486
• 关键词: DNA replication; cancer risk; fibroblasts; genetic markers; leukocyte activation /transformation; lymphoblast; neoplasm /cancer genetics; neoplastic cell; tissue /cell culture

The objectives of this project are to provide essential biological substrates required for the study of genetic susceptibility to cancer. The offeror will initiate transformation of lymphocytes with Epstein-Barr virus in order to establish long term cultures of lymphoblasts. These cells will subsequently be used to extract DNA for genetic marker studies. DNA will also be extracted directly from the granulocytes and lymphocytes in whole blood, from cultured fibroblasts and tumors, and from fresh tumor material. The DNAs obtained from these assays will be used by investigators in the Environmental Epidemiology Branch (Family Studies Section) and the Clinical Epidemiology Branch (Clinical Genetics Section) in ongoing studies of cancer susceptibility due to genetic etiology.

该项目的目标是提供基本的生物学 研究遗传易感性癌症所需的底物。 要约人将用爱泼斯坦 - 巴尔（Epstein-Barr）启动淋巴细胞转化 病毒以建立长期的淋巴细胞培养物。 这些 细胞随后将用于提取DNA进行遗传标记研究。 DNA还将直接从粒细胞和淋巴细胞中提取 在全血中，来自培养的成纤维细胞和肿瘤以及新鲜肿瘤 材料。 从这些测定中获得的DNA将由 环境流行病学分支的研究者（家庭研究 部分）和临床流行病学分支（临床遗传学部分） 在正在进行的遗传病因癌敏感性的研究中。