EICOSANOIDS AND PREGNANT HUMAN MYOMETRIUM

类花生酸与孕妇子宫肌层

• 批准号: 3327558
• 负责人: Ch V RAO
• 金额: $ 13.04万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3327558
• 关键词: birth; eicosanoid metabolism; hormone regulation /control mechanism; human tissue; isomerase; leukotrienes; lipoxygenase; microscopy; muscle contraction; myometrium; pregnancy; prostaglandin E; prostaglandin F; prostaglandin endoperoxide synthase; prostaglandin receptor; prostaglandins; western blottings

Eicosanoids play a fundamental role in the regulation of myometrial contractions. While some eicosanoids stimulate uterine contractions, others can inhibit them. The complex interplay between these two groups of eicosanoids is a likely determinant of the tone of the uterus. Receptor binding is a prerequisite for eicosanoids action, but whether these receptors are also present in pregnant myometrium and whether they change during pregnancy and labor has not been investigated. Eicosanoid action require not only their receptors but also their production. Both of these may very well be regulated by other hormones present during pregnancy. Finally, the mechanism(s) of eicosanoids action after receptor binding are unclear. Using the combination of biochemical, molecular and cell biological techniques, we will investigate myometrial changes of PGF, PGF2 alpha, PGI2 and LTC4 receptors, cyclooxygenase, 5-lipoxygenase, PGE2, PGI2 isomerases and PGF2 alpha synthase during pregnancy, preterm and term labor. In addition, regulation of pregnant human myometrial eicosanoid synthesizing enzymes, eicosanoids production and eicosanoid receptors by hormones present during pregnancy and the mechanism(s) of eicosanoids actions will also be investigated. Virtually none of the aspects of eicosanoids productions and binding discussed in this proposal have been studied in the pregnant human myometrium. The data is expected to advance our knowledge of the role and mechanism(s) of eicosanoids actions which should help to better understand mechanism(s) of initiation of labor at term and preterm. Better understanding can suggest more effective methods of delaying preterm labor and elective induction of labor at term. The delay or prevention of preterm labor can potentially save a large segment of the health care dollars now used for intensive neonatal care.

类二十烷酸在调节子宫肌层中发挥着重要作用 宫缩。 虽然一些类二十烷酸会刺激子宫收缩， 其他人可以抑制它们。 这两组之间复杂的相互作用 类二十烷酸可能是子宫张力的决定因素。 受体 结合是类二十烷酸作用的先决条件，但这些是否 受体也存在于怀孕的子宫肌层中以及它们是否发生变化 怀孕和分娩期间尚未进行调查。 类二十烷酸作用 不仅需要它们的受体，还需要它们的产生。 这两个 很可能受到怀孕期间存在的其他激素的调节。 最后，类二十烷酸在受体结合后的作用机制是 不清楚。 利用生化、分子和细胞的结合 生物技术，我们将研究PGF、PGF2的子宫肌层变化 α、PGI2 和 LTC4 受体、环氧合酶、5-脂氧合酶、PGE2、PGI2 怀孕、早产和足月期间的异构酶和 PGF2 α 合酶 劳动。 此外，调节妊娠人子宫肌层类二十烷酸 合成酶、类二十烷酸的产生和类二十烷酸受体 怀孕期间存在的激素和类二十烷酸的机制 行动也将受到调查。 几乎没有一个方面 本提案中讨论的类二十烷酸的生产和结合已 在怀孕的人类子宫肌层中进行了研究。 数据有望提前 我们对类二十烷酸的作用和机制的了解 应有助于更好地理解分娩开始的机制 足月和早产。 更好的理解可以提出更有效的方法 延迟早产和选择性足月引产。 这 延迟或预防早产可能会挽救很大一部分人的生命 目前用于重症新生儿护理的医疗保健资金的一部分。