VITAMIN D METABOLISM--FUNCTION OF KIDNEY DONOR/RECIPIENT

维生素 D 代谢——肾脏供体/受体的功能

• 批准号: 3068465
• 负责人: CONNIE W BALES
• 金额: $ 5.77万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3068465
• 关键词: aging; animal old age; human subject; juvenile animal; kidney hyperplasia; kidney metabolism; kidney transplantation; laboratory mouse; laboratory rat; mature animal; nephrectomy; nutrition of aging; nutrition related tag; physiologic stressor; tissue donors; vitamin D; vitamin metabolism

The proposed research will examine the impact of aging on the ability of the kidney to recover 25(OH)D-1 alpha-hydroxylase activity following uninephrectomy or renal transplantation. Preliminary studies in our laboratory indicate that remnant kidneys (postuninephrectomy) in young animals exhibit a remarkable ability to compensate for decreased renal mass by means of a rapid and dramatic increase in renal 25(OH)D-1 alpha- hydroxylase activity; this compensatory response appears to be limited or absent in aged kidneys. The objective of the planned experiments is to further characterize the compensatory response of enzyme activity post-uninephrectomy and to explore the effects of age on this phenomenon. Future studies will apply the results of these experiments to studies of human donors and transplant recipients of various ages. In addition, a rat model system of renal transplantation will be developed in order to examine the effects of age of isograft, as well as recipient, on 25(OH)D-1 alpha-hydroxylase activity. The results of this research will provide important data essential for the development of therapeutic procedures in kidney donors and transplant recipients which preserve vitamin D activity and minimize subsequent bone loss.

拟议的研究将探讨衰老对 肾脏恢复 25(OH)D-1 α-羟化酶的能力 单肾切除术或肾移植后的活动。 我们实验室的初步研究表明，残余物 幼年动物的肾脏（肾切除术后）表现出 通过以下方式补偿肾质量下降的显着能力 肾 25(OH)D-1 α- 快速且显着增加的手段 羟化酶活性；这种补偿性反应似乎是 老年肾脏中有限或不存在。 计划的目标 实验是为了进一步表征补偿反应 单肾切除术后酶活性的变化并探讨 年龄对这种现象的影响。 未来的研究将应用 这些实验的结果对人类捐赠者的研究和 不同年龄的移植受者。 此外，还建立了大鼠模型 肾移植系统将得到发展 检查同种移植物年龄以及受体的影响 25(OH)D-1 α-羟化酶活性。 这样做的结果 研究将提供重要的数据 肾脏捐赠者治疗程序的发展和 保留维生素 D 活性的移植受者 最大限度地减少随后的骨质流失。