FETAL SKIN BIOLOGY

胎儿皮肤生物学

• 批准号: 3314654
• 负责人: Karen A. Holbrook
• 金额: $ 25.22万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3314654
• 关键词: amniotic fluid; biological transport; biopsy; chromatography; collagen; collagenase; congenital skin disorder; disease /disorder proneness /risk; early embryonic stage; elastin; electron microscopy; embryo /fetus; enzyme mechanism; freeze etching; gene expression; gestational age; growth /development; histochemistry /cytochemistry; hormone regulation /control mechanism; human fetus tissue; human tissue; immunofluorescence technique; inborn metabolism disorder; intercellular connection; keratin; keratinization; lipid biosynthesis; mammalian embryology; mucopolysaccharides; postmortem; prenatal diagnosis; protein biosynthesis; protein structure; radiotracer; scanning electron microscopy; skin; skin disorder; skin disorder diagnosis; tissue /cell culture; tissue /cell preparation

This is a large multidisciplinary, collaborative program in which the primary objectives of the research are to expand our understanding of the structure, biochemistry and function of normal human embryonic and fetal skin during development, and to determine how the normal properties are altered antenatally by inherited skin disease. Studies are planned in three categories: 1) those in which we will continue to characterize the development of skin (epidermis, dermis and dermal-epidermal junction) in terms of various structural and biochemical markers of differentiation, 2) those which will provide insight into dermal-epidermal interactions that may influence the differentiation of the two tissues and the morphogenesis of the epidermis, and 3) those related to specific clinical studies. Studies in the first category include assays for age-related expression of involucrin, EGF-receptors, cell surface glycoconjugates and type V collagen by epidermal keratinocytes, and of antigens and receptors by Langerhans cells; of type VII collagen correlated with the appearance of anchoring fibrils at the DEJ; and of elastin and GAGs in the dermis. Morphometric studies of the epidermal cell surface are planned to increase our understanding of a possible role of the skin in transport during early gestation. The structure and biochemistry of premature skin will be studied and computer-assisted reconstruction will be used to evaluate the development of nerve/vessel networks of the skin. Cell culture, organ culture, artificial matrix cultures and grafts of fetal skin to the nude will be systems employed to evaluate dermal-epidermal interactions in tissue recombinant experiments. The clinical studies are focused on the evaluation of skin samples obtained at fetos-copy, and from newborns affected with inherited skin disease, studies of adult patients with ectodermal dysplasias; biochemical characterization of "fresh" amniotic fluid cells; studies of healing wounds from fetuses biopsied in utero; and examination of tissue from fetuses and newborns exposed to isotretinoin in utero (Vitmin A teratology). A consortium agreement has been planned to continue studies of lipid expression and biosynthesis (in culture) in embryonic, fetal and premature skin.

这是一个大型的多学科合作项目，其中 这项研究的主要目标是扩大我们对 正常人胚胎和胎儿的结构、生物化学和功能 发育过程中的皮肤，并确定正常特性如何 产前因遗传性皮肤病而改变。 研究计划于 三个类别：1）我们将继续描述的类别 皮肤（表皮、真皮和真皮-表皮交界处）的发育 各种分化结构和生化标记的术语，2) 那些将提供对真皮-表皮相互作用的深入了解 可能影响两种组织的分化和形态发生 表皮的部分，3) 与特定临床研究相关的部分。 第一类研究包括年龄相关表达的测定 外皮蛋白、EGF 受体、细胞表面糖复合物和 V 型胶原蛋白 由表皮角质形成细胞以及朗格汉斯的抗原和受体 细胞； VII 型胶原蛋白的含量与锚定的出现相关 DEJ处的原纤维；以及真皮中的弹性蛋白和 GAG。 形态测量 表皮细胞表面的研究计划增加我们的 了解皮肤在早期运输中可能发挥的作用 妊娠。 早产儿皮肤的结构和生物化学将是 研究和计算机辅助重建将用于评估 皮肤神经/血管网络的发育。 细胞培养、器官 培养、人工基质培养和胎儿皮肤裸体移植 将用于评估真皮-表皮相互作用的系统 组织重组实验。 临床研究主要集中在 评估胎儿镜检查和新生儿的皮肤样本 受遗传性皮肤病影响，对成年患者的研究 外胚层发育不良; “新鲜”羊膜的生化特征 液体细胞；子宫内活检胎儿伤口愈合的研究；和 对暴露于异维A酸的胎儿和新生儿的组织进行检查 子宫（维生素 A 畸胎学）。 已计划达成一项财团协议 继续研究脂质表达和生物合成（在培养物中） 胚胎、胎儿和早产儿皮肤。