Live cell super-resolution microscopy assessment of novel ratiometric luminescent transition metal complexes.

新型比率发光过渡金属配合物的活细胞超分辨率显微镜评估。

• 批准号: EP/R043191/1
• 负责人: Robert Pal
• 金额: $ 0.2万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FR043191%2F1
• 关键词: Live; cell; super; resolution; microscopy

This proposal is aimed at strengthening and providing the all-important next milestone in the current ongoing collaboration between the applicant and Dr. Filip Kielar, Naresuan University, Phitsanulok, Thailand. The collaboration is based on the Dr. Filip Kielar's expertise in the field of transition metal complexes and the expertise of Dr. Robert Pal in optical microscopy and associated live cell imaging and time-resolved spectroscopy. Due to the unique nature of transition metal complexes, such as longer fluorescent lifetime as opposed to short lived tissue auto-fluorescence and their potential application as photoinduced singlet oxygen generators, their in cellulo application is of great interest. We propose to study these newly synthesized compounds that can report on key biologically important analytes in live-cell studies using a variety of optical techniques, such as Laser Scanning Confocal-, Time-resolved- and Fluorescent Life Time imaging Microscopy.This collaboration was initially established as a result of a Royal Society International Exchange Scheme grant in 2014 that was awarded to Dr. Pal and Dr Kielar allowing them to engage in collaborative work. The scheme, being a travel only grant, allowed them to conduct a 2 week long visit each, facilitating all-important face to face meetings and discussions to shape and drive the project forward maximising scientific output and delivering under and post graduate lectures. Exactly one year and lots of hard work later we were in the fortunate positon, that our collective findings have been published in Dalton Transactions (2016, 45, 17420). As a result of this well-oiled collaboration a further more extensive Thai Research Fund grant was submitted in 12/2016 to facilitate the synthesis of the next generation of complexes to be studied at Durham (awarded to Dr. Kielar, commenced 02/2017 with no travel allowance for the PI). However, the above mentioned grant only facilitates research staff (3 year PhD) and consumables cost to be met at Naresuan University. Dr. Pal has already earmarked the continuation of this project as a dedicated 2017/18 MSc. student.Researchers at Narressuan University are extremely happy with our preliminary fruitful collaborative work and communications on this subject, and truly believe that due to the expertise and dedication at Durham we have achieved so much in so little time on this subject despite the logistics originating from our very different geological and financial position.

该提案旨在加强申请人与泰国彭世洛那黎宣大学 Filip Kielar 博士当前正在进行的合作，并为其提供最重要的下一个里程碑。此次合作基于 Filip Kielar 博士在过渡金属配合物领域的专业知识以及 Robert Pal 博士在光学显微镜及相关活细胞成像和时间分辨光谱学方面的专业知识。由于过渡金属配合物的独特性质，例如与短寿命的组织自发荧光相比具有更长的荧光寿命以及它们作为光诱导单线态氧发生器的潜在应用，它们在纤维素中的应用引起了极大的兴趣。我们建议研究这些新合成的化合物，这些化合物可以使用各种光学技术（例如激光扫描共焦、时间分辨和荧光寿命成像显微镜）报告活细胞研究中具有重要生物学意义的关键分析物。该项目是 2014 年皇家学会国际交流计划拨款的结果，该拨款授予 Pal 博士和 Kielar 博士，使他们能够从事协作工作。该计划是一项仅限旅行的补助金，允许他们每人进行为期两周的访问，促进所有重要的面对面会议和讨论，以塑造和推动项目向前发展，最大限度地提高科学产出，并提供研究生和研究生讲座。整整一年，经过大量的努力，我们很幸运，我们的集体研究结果发表在 Dalton Transactions (2016, 45, 17420) 上。由于这次合作顺利，泰国研究基金于 2016 年 12 月提交了更广泛的资助，以促进在达勒姆研究的下一代复合物的合成（授予 Kielar 博士，于 2017 年 2 月开始） PI 没有差旅津贴）。然而，上述补助金仅用于资助Naresuan大学的研究人员（3年博士）和消耗品费用。 Pal 博士已指定继续该项目作为 2017/18 年度专门的理学硕士项目。纳瑞苏安大学的研究人员对我们在这一主题上的初步富有成效的合作和交流感到非常满意，并坚信，由于达勒姆大学的专业知识和奉献精神，我们在如此短的时间内在这一主题上取得了如此多的成就，尽管后勤工作来自于我们的地质和财务状况截然不同。

项目成果

Synthesis and investigation of a tris-cyclometalated iridium complex bearing a single quarternary ammonium group

含单一季铵基团的三环金属化铱配合物的合成与研究

• DOI: 10.1016/j.ica.2019.119066
• 发表时间: 2019-11-01
• 期刊: Inorganica Chimica Acta
• 影响因子: 2.8
• 作者: S. Meksawangwong;B. Gohil;Wikorn Punyain;R. Pal;F. Kielar
• 通讯作者: F. Kielar