A National Network for Applications of High-Field NMR in the Life and Physical Sciences

高场核磁共振在生命和物理科学中应用的国家网络

• 批准号: EP/R030065/1
• 负责人: Dusan Uhrin
• 金额: $ 174.31万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FR030065%2F1
• 关键词: National; Network; Applications; Field; NMR

Nuclear Magnetic Resonance (NMR) spectroscopy is a very powerful technique used in many fields of physical and life sciences, including all branches of chemistry, material sciences and biology. It provides wealth of information about (bio)molecules in solution and materials and insoluble molecules in solid state.Increasing the operating frequency of NMR spectrometers leads to higher sensitivity and resolution. Both are required when working with small (tens of micrograms) amounts of sample, or with large biomacromolecules (proteins, DNA, carbohydrates). Similarly, and especially for some nuclei, solid- state NMR accrues additional benefits at higher frequencies. There are therefore instances where only high-field spectrometers can solve a particular problem. However, very-high field and ultra-high field spectrometers come at a cost, largely associated with the development and production of their superconducting magnets. It is therefore imperative that such instrumentation is used efficiently through sharing by many research groups. Modern NMR spectrometers are versatile pieces of equipment that can be customised to tackle a range of samples, so sharing across disciplines and applications (e.g. liquid- vs solid-state) is possible. Spectroscopists from across the UK working in a wide-range of scientific disciplines have therefore agreed to work towards a coordinated strategy for sharing very-high and ultra-high field NMR equipment to underpin fundamental and applied sciences in a wide range of areas, ranging from battery and solar panel research, enzyme catalysis, drug discovery, food security, understanding disease or characterisation of environmental matrices. By sharing technologically advanced methodologies, the impact on the UK society will be maximised, both in the academia and industry, thus contributing to the UK economic viability and overall well-being.As part of this strategy, Scottish NMR researchers representing all Scottish universities and the CRUK Beatson Institute, have decided to establish Scottish High Field (SHF) NMR Centre around the upgraded 800 MHz NMR spectrometer housed in the SoC at UoE. This upgrade will equip the spectrometer with up-to-date NMR capabilities for liquid- and solid-state experiments. This involves installation of two CryoProbes, MAS controller, high power 1H amplifier and three solid-state probes in Year 1, followed by the upgrade of the console in Year 3 to guarantee continued operation of the Centre beyond the duration of the grant.Equipping this spectrometer with the latest technology and utilising the existing magnet is the most economical option that will fulfil our aim: to provide cutting edge support in liquid- and solid-state NMR to physical and life sciences researchers to deliver significant academic and industrial impact. The measurement time on this upgraded 800-MHz liquids/solid-state NMR spectrometer will be shared between researchers from all Scottish universities, CRUK Beatson Institute and UoE researchers. Sustained operation of the Centre will be guaranteed by the financial support of participating institutions and umbrella organisations of life and chemical sciences in Scotland, SULSA and ScotCHEM. Importantly, the Centre will be operated by staff from all participating institutions.Using the Centre as the focal point, a hub and spoke model will be used to manage access to the mid- to high-magnetic field NMR spectrometers in Scotland. This will optimise access to NMR equipment and facilitate access to ultra-high field spectrometers in England, foster day-to-day cooperation, ensure exchange of information and dissemination of best practise.The SHF NMR Centre will transform the way high-field NMR is applied to chemistry, biology, environmental and material sciences in academia and industry. It will form a platform for interactions with other regional and national very-high field and ultra-high field UK NMR centres.

核磁共振 (NMR) 波谱是一种非常强大的技术，应用于物理和生命科学的许多领域，包括化学、材料科学和生物学的所有分支。它提供了有关溶液和材料中的（生物）分子以及固态不溶性分子的丰富信息。提高 NMR 波谱仪的工作频率可带来更高的灵敏度和分辨率。当处理少量（数十微克）样品或大型生物大分子（蛋白质、DNA、碳水化合物）时，两者都是必需的。同样，特别是对于某些原子核，固态核磁共振在较高频率下会带来额外的好处。因此，在某些情况下，只有高场光谱仪才能解决特定问题。然而，极高场和超高场光谱仪是有成本的，很大程度上与其超导磁体的开发和生产有关。因此，许多研究小组必须通过共享来有效地使用此类仪器。现代核磁共振波谱仪是多功能设备，可以进行定制以处理各种样品，因此可以跨学科和应用（例如液态与固态）共享。因此，来自英国各地从事广泛科学学科工作的光谱学家同意致力于制定一项协调战略，共享甚高和超高场核磁共振设备，以支持广泛领域的基础科学和应用科学，包括：电池和太阳能电池板研究、酶催化、药物发现、食品安全、了解疾病或环境基质表征。通过分享技术先进的方法，对英国社会在学术界和工业界的影响将最大化，从而为英国的经济活力和整体福祉做出贡献。作为该战略的一部分，代表所有苏格兰大学和大学的苏格兰 NMR 研究人员CRUK Beatson 研究所决定围绕 UoE SoC 中升级的 800 MHz NMR 波谱仪建立苏格兰高场 (SHF) NMR 中心。此次升级将为光谱仪配备最新的 NMR 功能，用于液态和固态实验。这包括在第一年安装两个 CryoProbe、MAS 控制器、高功率 1H 放大器和三个固态探头，然后在第三年升级控制台，以保证该中心在拨款期限后继续运行。采用最新技术并利用现有磁铁的光谱仪是实现我们目标的最经济选择：为物理和生命科学研究人员提供液态和固态 NMR 领域的尖端支持，以产生重大的学术和工业影响。这款升级版 800 MHz 液体/固态 NMR 波谱仪的测量时间将由苏格兰所有大学、CRUK Beatson 研究所和苏格兰联合大学研究人员共享。该中心的持续运作将得到苏格兰、SULSA 和 ScotCHEM 生命和化学科学参与机构和伞式组织的财政支持。重要的是，该中心将由所有参与机构的工作人员运营。以该中心为焦点，将使用轮辐模型来管理对苏格兰中高磁场核磁共振波谱仪的访问。这将优化 NMR 设备的获取并促进在英国使用超高场谱仪，促进日常合作，确保信息交流和传播最佳实践。SHF NMR 中心将改变高场 NMR 的方式应用于学术界和工业界的化学、生物学、环境和材料科学。它将形成一个与其他地区和国家甚高场和超高场英国核磁共振中心互动的平台。

项目成果

Dermacozine N, the First Natural Linear Pentacyclic Oxazinophenazine with UV-Vis Absorption Maxima in the Near Infrared Region, along with Dermacozines O and P Isolated from the Mariana Trench Sediment Strain Dermacoccus abyssi MT 1.1T

Dermacozine N 是第一个在近红外区域具有 UV-Vis 吸收最大值的天然线性五环恶嗪吩嗪，以及从马里亚纳海沟沉积物菌株 Dermacoccus abyssi MT 1.1T 中分离出的 Dermacozine O 和 P

• DOI: http://dx.10.3390/md19060325
• 发表时间: 2021
• 期刊: Marine Drugs
• 影响因子: 5.4
• 作者: Juhasz B

SHARPER-enhanced benchtop NMR: improving SNR by removing couplings and approaching natural linewidths.

SHARPER 增强型台式 NMR：通过消除耦合和接近自然线宽来提高 SNR。

• DOI: http://dx.10.1039/d2cc01325h
• 发表时间: 2022
• 期刊: Chemical communications (Cambridge, England)
• 作者: Dickson CL

Monitoring off-resonance signals with SHARPER NMR - the MR-SHARPER experiment.

使用 SHARPER NMR 监测非共振信号 - MR-SHARPER 实验。

• DOI: http://dx.10.1039/d2an00134a
• 发表时间: 2022
• 期刊: The Analyst
• 作者: Davy M

Methane emissions and rumen metabolite concentrations in cattle fed two different silages

饲喂两种不同青贮饲料的牛的甲烷排放和瘤胃代谢物浓度

• DOI: http://dx.10.1038/s41598-022-09108-w
• 发表时间: 2022
• 期刊: Scientific Reports
• 影响因子: 4.6
• 作者: Bica R

Molecular level study of hot water extracted green tea buried in soils - a proxy for labile soil organic matter.

埋在土壤中的热水提取绿茶的分子水平研究 - 不稳定土壤有机质的代表。

• DOI: http://dx.10.1038/s41598-020-58325-8
• 发表时间: 2020
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Bell NGA