STRATOSPHERIC OZONE DEPLETION AND INCREASED DNA DAMAGE

平流层臭氧消耗和 DNA 损伤加剧

• 批准号: 3549160
• 负责人: RONALD D LEY
• 金额: $ 11.32万
• 依托单位: LOVELACE RESPIRATORY RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3549160
• 关键词: DNA; biopsy; environmental health; environmental radiation; gel electrophoresis; human subject; melanins; neoplasm /cancer epidemiology; ozone; physical chemical interaction; pyrimidine dimers; radiation genetics; radiation related neoplasm /cancer; radiation sensitivity; skin neoplasms; ultraviolet radiation

Depletion of stratospheric ozone will lead to an increase in solar ultraviolet radiation (UVR) reaching the Earth's surface. The type of UVR that will increase is the so-called UV-B radiation (280-320 nm) which is known to be biologically damaging. The increased levels of UVR will undoubtedly result in an increase in the number of human skin cancers. Based on results of previous animal carcinogenesis studies, it should be possible to use DNA damage as a biochemical marker for use in epidemiological studies to predict increased levels of skin cancer associated with decreased levels of stratospheric ozone. The information obtained from the proposed research will be essential in determining increased levels of epidermal DNA damage in individuals of various skin types as a function of a given ozone depletion. Specifically, we wish to determine: 1) the effectiveness of wavelengths in the UV-B region in inducing DNA damage in the skin of individuals of skin types I-V as classified by sensitivity to the sun and tanning response; and, 2) use solar simulators to mimic UVR transmission associated with various levels of ozone depletion to determine whether the action spectra generated in aim 1 can, in fact, be used to predict increased levels of DNA damage that will occur as a result of ozone depletion.

平流臭氧的耗竭将导致太阳能增加 到达地球表面的紫外线辐射（UVR）。 类型 UVR的增加是所谓的UV-B辐射（280-320 NM），已知在生物学上具有破坏性。 增加 UVR的水平无疑会导致数量增加 人类皮肤癌。 基于先前动物的结果 致癌研究，应该有可能使用DNA损伤 作为用于流行病学研究的生化标记 预测与降低相关的皮肤癌水平增加 平流层臭氧的水平。 从拟议的研究中获得的信息将是 确定表皮DNA水平升高至关重要 各种皮肤类型的个体的损害与 给定臭氧消耗。 具体来说，我们希望确定：1） 在诱导DNA中波长在UV-B区域的有效性 由I-V类型皮肤的皮肤损害由 对太阳和晒黑反应的敏感性； 2）使用太阳能 模拟器以模拟与各种相关的UVR传输 臭氧耗竭的水平以确定作用光谱是否 实际上，在AIM 1中生成的生成可以用来预测增加 臭氧会导致的DNA损伤水平 消耗。