ELECTRICAL APPROACH FOR IN VITRO TOXICOLOGY

体外毒理学的电气方法

• 批准号: 3496752
• 负责人: CHARLES R KEESE
• 金额: $ 5万
• 依托单位: APPLIED BIOPHYSICS, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3496752
• 关键词: alternatives to animals in research; biosensor device; cell adhesion; cell cell interaction; cell cycle; cell migration; cell motility; clone cells; cytotoxicity; electrical impedance; electrical measurement; electrodes; method development; morphology; statistics /biometry; tissue /cell culture; toxicant screening

We have been developing a new means to electrically study animal cells grown in tissue culture and intend to make a commercial device suitable for use in the medical/biological research and testing market. The instrumental method, referred to as ECIS (electric cell-substrate impedance sensor), is a non-invasive technique that measures several parameters related to cell behavior, some of which have previously been difficult to quantify. These include adhesion and spreading, cell growth, cell motility, and the barrier function of cell monolayers. The objective of the proposed research is to test the feasibility of using this approach to evaluate the cytotoxicity of compounds. The cell behaviors detected by ECIS are complex and, hence, require the integrated activities of many cellular systems. It is hoped their measurement will be particularly useful in screening potentially toxic compounds without the use of whole animals. Specifically, extensive ECIS measurements will be carried out using different cell lines exposed to varying concentrations of compounds with well documented cytotoxicity. These dose-response data will be statistically compared with existing data both from established cell culture assays and from whole animals. This aspect of the research will be carried out in collaboration with the Center for Alternatives to Animal Testing at Johns Hopkins University.

我们一直在开发一种新的手段来电气研究动物细胞 在组织培养中生长，并打算使商业设备适合 在医学/生物学研究和测试市场中使用。 这 仪器方法，称为ECI（电细胞基底阻抗） 传感器），是一种测量几个参数的非侵入性技术 与细胞行为有关，其中一些以前很难 量化。 这些包括粘附和扩散，细胞生长，细胞 运动性和细胞单层的屏障功能。 拟议研究的目的是测试使用的可行性 这种评估化合物细胞毒性的方法。 细胞 ECI检测到的行为很复杂，因此需要集成 许多细胞系统的活动。 希望他们的测量能 在筛选潜在有毒化合物的情况下特别有用 全动物的使用。 具体而言，将使用大量的ECIS测量 暴露于不同浓度的化合物的不同细胞系 文献良好的细胞毒性。 这些剂量反应数据将是 从统计上与现有数据进行比较，都来自已建立的单元格 培养测定和全动物。 研究的这一方面将是 与动物替代中心合作进行 约翰·霍普金斯大学的测试。