MINIATURIZED BENCH-TOP ANALYTICAL CELL SORTER

小型台式分析细胞分选机

• 批准号: 3492741
• 负责人: PERRY R SKEATH
• 金额: $ 4.47万
• 依托单位: SPRAYCHIP SYSTEMS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-10 至 1992-01-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3492741
• 关键词: biomedical equipment development; biotechnology; cell sorting; clinical biomedical equipment; diagnosis design /evaluation; flow cytometry; fluid flow; fluorescence; heavy metals; microscopy; plastics; semiconduction; silicon; suspensions

The purpose of this work is to develop an instrument which integrates flow cytometry, cell sorting, and image cytometry. The system sorts cells in a liquid medium using fluorescence cytometry. The system then presents the selected cells for microscopic examination. The technological innovation consists of (1) a single assembly which identifies, sorts, and accumulates selected cells (2) the entire device is compact so that it may be mounted directly on a microscope stage. The ability to sort and accumulate cells of interest in the field of the microscope for image analysis can greatly expedite the evaluation of a specimen. This would be useful in many types of tumor analysis including lymphomas, Hodgkin's disease, myeloma, hair cell leukemia, and mycosis fungoids.

这项工作的目的是开发一种整合流量的工具 细胞仪，细胞分类和图像细胞仪。 该系统将单元格在A中 使用荧光细胞仪的液体培养基。 然后系统提出 选定的细胞进行微观检查。 技术创新 由（1）单个组件组成，该组件识别，分类和累积 选定的单元格（2）整个设备都紧凑，以便可以安装 直接在显微镜阶段。 分类和积累细胞的能力 在显微镜领域感兴趣的图像分析可以极大地 加快标本的评估。 这在许多类型中都会有用 肿瘤分析包括淋巴瘤，霍奇金病，骨髓瘤，头发 细胞白血病和真菌病真菌。