CELLULAR MECHANISMS IN THE IMMUNE RESPONSE

免疫反应中的细胞机制

• 批准号: 3480479
• 负责人: RICHARD W DUTTON
• 金额: $ 32.43万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3480479
• 关键词: B lymphocyte; antigens; athymic mouse; autoradiography; cell cell interaction; cell differentiation; cellular immunity; embryo /fetus; gel electrophoresis; gene expression; genetic library; genetic recombination; genetic translation; genetically modified animals; helper T lymphocyte; histocompatibility antigens; hybridomas; immunofluorescence technique; immunogenetics; immunoglobulin genes; immunoglobulins; immunoregulation; interleukin 2; laboratory mouse; laboratory rat; leukocyte activation /transformation; lymphokines; monoclonal antibody; nucleic acid probes; protein sequence; protein structure function; radiotracer; receptor; tissue /cell culture

Studies are proposed in four areas of cellular and molecular immunology. 1. Studies on a T helper stimulatory factor, THSF, a new lymphokine described by us in the previous grant period. THSF is derived from a cloned thymic epithelial cell line and appears to act on peripheral L3T4 T cells to coinduce the synthesis of lL2. (a) The factor will be purified to homogeneity and the cDNA coding for THSF will be identified and sequenced. (b) The purified factor will be used in receptor ligand studies. (c) A biological study will be carried out to determine the number of cell types upon which THSF acts and the precise nature of its effect. 2. Two lines of analysis are proposed to investigate the nature of T cell-B cell interactions, one, direct visualization studies with living stained cells, two, an analysis of T cell-B cell interactions in B cell differentiation. 3. Studies on the role antigen in the differentiation phase of the B cell response analysed with cells from transgenic mice expressing rearranged immunoglobulin genes with cells expressing the V-ese gene in the B cell response to High-SRBC. 4. The development of an experimental model to investigate the role of T cells and T cell products in the regulation of B cells isotypes.

提出了细胞和分子四个领域的研究 免疫学。 1. 新型辅助T刺激因子THSF的研究 我们在上一个资助期间描述的淋巴因子。 THSF 是 源自克隆的胸腺上皮细胞系，似乎 作用于外周 L3T4 T 细胞，共同诱导 lL2 的合成。 (a) 因子将被纯化至同质并且 cDNA THSF 的编码将被识别并测序。 (b) 纯化的因子将用于受体配体研究。 (c) 将进行生物学研究以确定数量 THSF 作用的细胞类型及其确切性质 影响。 2. 提出两条分析路线来研究 T 细胞-B 细胞相互作用，一，直接可视化研究 活染色细胞，二，T 细胞-B 细胞相互作用分析 在B细胞分化中。 3. 抗原在细胞分化阶段的作用研究 使用转基因小鼠的细胞分析 B 细胞反应 表达重排的免疫球蛋白基因，细胞表达 B 细胞对 High-SRBC 反应中的 V-ese 基因。 4. 开发实验模型来研究 T细胞和T细胞产物在B细胞调节中的作用 同种型。