VIRAL AND CELLULAR GENE EXPRESSION AND GROWTH REGULATION

病毒和细胞基因表达和生长调节

• 批准号: 3479444
• 负责人: Fred T Valentine
• 金额: $ 71.94万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-06-01 至 1993-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3479444
• 关键词: AIDS; Kaposi's sarcoma; Polyomavirus; cell growth regulation; chromosome deletion; chromosome disorders; complementary DNA; cytogenetics; gene expression; genetic enhancer element; genetic library; genetic manipulation; genetic promoter element; genetic recombination; genetic transcription; human tissue; laboratory mouse; molecular cloning; natural gene amplification; oncogenes; plasmids; tissue /cell culture; transfection; viral carcinogenesis; virus DNA; virus genetics

The main objectives of this proposal are: 1) The study of the DNA rearrangements which are promoted by polyoma virus elements in transformed cells, and the comparison of these rearrangements with similar phenomena controlled by cellular events. 2) The study of the regulation of transcription of integrated polyoma virus genomes by cis DNA elements and transactivatinf or transrepressing proteins. 3) The cloning and characterization of genes controlling cell cycle progression in animal cells, as defined by ts mutants of the BHK hamster cell line specifically blocked in cell cycle progression. 4) The study of the control of transcription of specific cellular genes in the cycle of normal or virus transformed cells. 5) The study of the nature of Kaposi's Sarcoma in AIDS patients. Our main long range objective is to understanding of what patterns of regulation are altered in transformed cells. To this aim the studies of polyoma promoted gene rearrangements and of the expression of integrated polyoma genomes should shed light on the factors controlling, the expression of transforming genes, as well as on the effect that transforming gene-products have on the transcription of other cellular genes. The study of cell cycle genes and of the regulation of gene expression during the cell cycle should provide information on what differentiates the control of cell division of cancer cells from that of normal cells.

该提案的主要目标是： 1) 多瘤病毒促进DNA重排的研究 转化细胞中的元素，以及这些重排的比较 具有由细胞事件控制的类似现象。 2）整合型多瘤病毒转录调控研究 基因组由顺式 DNA 元件和反式激活或反式抑制蛋白组成。 3）细胞周期控制基因的克隆与表征 动物细胞中的进展，由 BHK 仓鼠的 ts 突变体定义 细胞系特异性阻断细胞周期进程。 4）特定细胞基因转录控制的研究 正常或病毒转化细胞的周期。 5）艾滋病患者卡波西肉瘤性质的研究。 我们的主要长期目标是了解什么模式 转化细胞中的调节发生改变。 为此目的，研究 多瘤促进基因重排和整合表达 多瘤基因组应该揭示控制因素 转化基因的表达，以及对转化基因的影响 转化基因产物对其他细胞的转录有影响 基因。 细胞周期基因及基因调控的研究 细胞周期期间的表达应提供有关什么的信息 区分癌细胞的细胞分裂的控制 正常细胞。