REGULATION OF K+ TRANSPORT IN VASCULAR SMOOTH MUSCLE

血管平滑肌中 K 运输的调节

• 批准号: 3473860
• 负责人: DAVID H WARDEN
• 金额: $ 9.52万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3473860
• 关键词: calcium flux; cardiovascular disorder; chloride channels; electrophysiology; hypertension; ion transport; laboratory rabbit; laboratory rat; muscle contraction; potassium channel; vascular smooth muscle; vasomotion

The subject of this proposal is the relationship between contraction of vascular smooth muscle and concurrent changes in potassium transport across the cell membrane. In particular, this investigation focuses on the specific ion transport processes responsible for the increase in potassium and chloride transport out of smooth muscle cells when they are stimulated to contract. The preliminary data indicate that the majority of this ion transport does not occur via ion channels. Instead, the predominant pathway for potassium efflux is dependent on the presence of chloride and inhibited by loop diuretics. These two features are strong evidence that this pathway represents some form of cotransport. Studies to identify and characterize this transport system are outlined. The functional importance of this ion transporter will be evaluated by assessing whether interruption of the system limits the ability of arteries to contract. The preliminary studies also demonstrate that there is a tight link between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium concentration and activity of the cotransport system. This objective includes an evaluation of potassium cotransport in cultured vascular smooth muscle cells. Achievement of this objective will set the stage for more detailed studies of the regulation of this ion transport system. The studies are important and relevant for two primary reasons. First, this investigation will advance our understanding of how monovalent ion transport systems participate in regulation of the vascular system. Second, these studies have immediate and direct bearing on our understanding and rationale for treatment of hypertension and other cardiovascular disease processes.

该提议的主题是收缩之间的关系 血管平滑肌和钾转运的同时变化 细胞膜。 特别是，这项调查的重点是 特定的离子运输过程负责增加钾 刺激时从平滑肌细胞中氯化物转运 收缩。 初步数据表明该离子的大多数 运输不会通过离子通道发生。 相反，主要是 钾外排的途径取决于氯化物的存在和 循环利尿剂抑制。 这两个特征是有力的证据表明 该途径代表了某种形式的共同运动。 识别和 概述了该运输系统的特征。 功能重要性 该离子转运蛋白将通过评估是否中断来评估 系统限制了动脉收缩的能力。 初步 研究还表明，细胞内存在紧密的联系 钾转运过程的钙和激活。 因此，决赛 这项研究的阶段将检查细胞内的关系 钾转运过程的钙和激活。 因此，决赛 这项研究的阶段将检查细胞内的关系 Cotransport系统的钙浓度和活性。 这 目标包括评估培养的钾共译 血管平滑肌细胞。 实现这一目标将设定 阶段进行更详细的研究该离子运输调节 系统。 这些研究很重要，有两个主要原因。 首先，这项调查将提高我们对单价如何的理解 离子运输系统参与血管系统的调节。 其次，这些研究立即与我们直接相关 理解和理由治疗高血压和其他 心血管疾病过程。