REGULATION OF K+ TRANSPORT IN VASCULAR SMOOTH MUSCLE

血管平滑肌中 K 运输的调节

基本信息

批准号：
3473860
负责人：
DAVID H WARDEN
金额：
$ 9.52万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-04-01 至 1996-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3473860
关键词：
calcium flux cardiovascular disorder chloride channels electrophysiology hypertension ion transport laboratory rabbit laboratory rat muscle contraction potassium channel vascular smooth muscle vasomotion

项目摘要

The subject of this proposal is the relationship between contraction of vascular smooth muscle and concurrent changes in potassium transport across the cell membrane. In particular, this investigation focuses on the specific ion transport processes responsible for the increase in potassium and chloride transport out of smooth muscle cells when they are stimulated to contract. The preliminary data indicate that the majority of this ion transport does not occur via ion channels. Instead, the predominant pathway for potassium efflux is dependent on the presence of chloride and inhibited by loop diuretics. These two features are strong evidence that this pathway represents some form of cotransport. Studies to identify and characterize this transport system are outlined. The functional importance of this ion transporter will be evaluated by assessing whether interruption of the system limits the ability of arteries to contract. The preliminary studies also demonstrate that there is a tight link between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium concentration and activity of the cotransport system. This objective includes an evaluation of potassium cotransport in cultured vascular smooth muscle cells. Achievement of this objective will set the stage for more detailed studies of the regulation of this ion transport system. The studies are important and relevant for two primary reasons. First, this investigation will advance our understanding of how monovalent ion transport systems participate in regulation of the vascular system. Second, these studies have immediate and direct bearing on our understanding and rationale for treatment of hypertension and other cardiovascular disease processes.

本提案的主题是收缩与收缩之间的关系血管平滑肌和钾转运的同时变化细胞膜。特别是，本次调查的重点是导致钾增加的特定离子传输过程当平滑肌细胞受到刺激时，氯离子会从平滑肌细胞中转运出来签订合同。初步数据表明，该离子的大部分运输不通过离子通道发生。相反，占主导地位的钾流出的途径取决于氯化物的存在和受袢利尿剂抑制。这两个特征有力地证明了该途径代表某种形式的协同转运。研究识别和概述了该运输系统的特征。功能重要性该离子转运蛋白的功能将通过评估是否中断来评估该系统的功能限制了动脉收缩的能力。初步的研究还表明，细胞内的钙和钾转运过程的激活。于是，最终的本研究阶段将检查细胞内钙和钾转运过程的激活。于是，最终的本研究阶段将检查细胞内钙浓度和共转运系统的活性。这目标包括对培养物中钾协同转运的评估血管平滑肌细胞。这一目标的实现将确定对该离子传输的调节进行更详细研究的阶段系统。这些研究之所以重要且相关，主要有两个原因。首先，这项研究将加深我们对单价如何离子转运系统参与血管系统的调节。其次，这些研究对我们的研究有直接的影响。治疗高血压和其他疾病的理解和原理心血管疾病过程。