IMMUNOBIOLOGICAL ROLE OF FERTILIZATION ANTIGEN (FA-1)

受精抗原 (FA-1) 的免疫生物学作用

• 批准号: 3469890
• 负责人: RAJESH K NAZ
• 金额: $ 11.41万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3469890
• 关键词: antigens; embryo implantation; female; fertility; fertility immunology; germ cells; human tissue; immunoaffinity chromatography; laboratory mouse; monoclonal antibody; protein sequence; sperm

We developed a germ-cell specific monoclonal antibody (MA-24) against spermatozoa plasma membrane (Naz et al., Science 225:342, 1984) which blocked human sperm penetration of zona-free hamster ova and inhibited in vitro fertilization of mouse ova by murine sperm without causing agglutination or immobilization of sperm. It recognized a single glycoprotein of testicular origin present on postacrosomes, midpieces and tails of sperm. This fertilization antigen (FA-1) has been isolated from human and murine germ cells using MA-24 immunoaffinity chromatography (Naz et al., Proc. Natl. Acad. Sci., USA, 83:5713, 1986). The overall objective of this project is to characterize sperm-specific antigen (FA-1) and investigate its role in fertilization, embryonic development, implantation and postimplantation fertility in mice. FA-1 will be isolated from murine testes in large amounts by using immunoaffinity chromatography and further purified and characterized for its molecular identity, subunit structure, amino acid contents and carbohydrate composition. The antigenic fragment (polypeptide) of the FA-1 will be isolated by using high performance immunoaffinity chromatography. FA-1 will be checked for its immunogenicity and monoclonal antibodies will be raised reacting against its various antigenic determinants. The fate of antigen after fertilization will be investigated. It will be checked whether MA-24 is directed against carbohydrate or polypeptide portion of the antigenic molecule. We will check the role of FA-1 in fertilization and post-fertilization fertility. We will investigate the cross-reaction of FA-1 with sera from immunoinfertile patients. Besides enabling us to define the molecular events underlying fertilization, embryonic development, and implantation, this project will provide a basis for specific methods for diagnosing and managing involuntary immunoinfertility in humans and development of an anti-sperm contraceptive vaccine.

我们开发了一种针对 精子质膜（Naz等，科学225：342，1984） 阻塞无Zona无Zona仓鼠OVA的人类精子渗透并抑制 小鼠精子对小鼠卵的体外受精而不会引起 精子的凝集或固定。 它认出了一个 乳突后，中型和睾丸来源的糖蛋白 精子的尾巴。 这种受精抗原（FA-1）已从 使用MA-24免疫亲和力色谱法（NAZ）的人和鼠类生殖细胞 等，proc。纳特。学院。美国科学，美国，83：5713，1986）。 总体目标 该项目的特征是精子特异性抗原（FA-1）和 研究其在受精，胚胎发育，植入中的作用 和小鼠植入后的生育能力。 FA-1将与鼠隔离 通过使用免疫亲和力色谱法和进一步的睾丸大量睾丸 纯化和特征以其分子身份，亚基结构， 氨基酸含量和碳水化合物组成。 抗原片段 FA-1的（多肽）将通过使用高性能隔离 免疫亲和力色谱。 FA-1将检查其免疫原性 和单克隆抗体将与其各种抗体作出反应 抗原决定因素。 受精后抗原的命运将是 调查。 将检查MA-24是否针对 抗原分子的碳水化合物或多肽部分。 我们将 检查FA-1在施肥和施肥后生育能力中的作用。 我们将研究FA-1与血清的交叉反应 免疫吸毒患者。 除了使我们定义分子 受精，胚胎发育和植入的事件， 该项目将为诊断和 管理人类的非自愿免疫传递和发展 抗植物避孕疫苗。