MOLECULAR POPULATION GENETICS OF ADH IN DROSPHILA

果蝇 ADH 的分子群体遗传学

基本信息

批准号：
3467683
负责人：
STEPHEN W SCHAEFFER
金额：
$ 8.3万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-07-01 至 1994-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3467683
关键词：
Drosophilidae aldehyde dehydrogenases animal population genetics biochemical evolution biological polymorphism gene expression gene frequency genetic manipulation genetic models genotype molecular genetics natural gene amplification natural selections nucleic acid sequence radiation genetics species difference

项目摘要

The objective of the proposed study is to analyze the intraspecific and interspecific variation of nucleotide sequences of a phenotypically monomorphic locus to determine the important evolutionary forces that generate and maintain nucleotide diversity in natural populations. Nucleotide diversity is the ultimate source of phenotypic variation which allows organisms to adapt to new environmental challenges in natural populations such as acquiring disease resistance. Thus, it is important to understand the evolutionary forces that modulate the levels of genetic variation in natural populations. In addition, the evolutionary dynamics of a genetic locus that is phenotypically simple must be understood before the evolutionary forces that shape the pattern and organization of nucleotide diversity of phenotypically complex loci can be ascertained. The proposed study will sample the nucleotide diversity of 100 nucleotide sequences of the alcohol dehydrogenase (Adh) region, a phenotypically monomorphic locus, from five natural populations of Drosophila pseudoobscura and one population of D. persimilis to test whether this locus fits the predictions of the neutral theory of molecular evolution. A phenotypically monomorphic locus should behave according to the predictions of the neutral theory in the absence of other evolutionary forces such as natural selection. Any departures from the predictions of the neutral model seen in the Adh region of D. pseudoobscura will suggest that the action of natural selection may shape the pattern and organization of nucleotide diversity at a phenotypically monomorphic locus.

拟议研究的目的是分析种内和核苷酸序列的种间变异表型单态位点以确定重要的产生和维持核苷酸多样性的进化力量在自然种群中。核苷酸多样性才是终极表型变异的来源，使生物体能够适应自然种群面临的新环境挑战，例如获得抗病能力。因此，了解这一点很重要调节遗传水平的进化力量自然种群的变异。此外，进化论表型简单的遗传位点的动力学必须是在塑造模式的进化力量之前就已被理解和表型复杂的核苷酸多样性的组织可以确定位点。拟议的研究将对 100 个核苷酸多样性进行采样乙醇脱氢酶 (Adh) 区域的核苷酸序列表型单态位点，来自五个自然种群 Drosophila pseudoobscura 和 D. persimilis 的一个种群检验该轨迹是否符合中性理论的预测分子进化论。表型单态基因座应该根据中性理论的预测表现缺乏其他进化力量，例如自然选择。任何偏离中立预测的情况在 D.pseudoobscura 的 Adh 区域中看到的模型表明自然选择的作用可能会塑造模式并表型上核苷酸多样性的组织单态基因座。