LIPID NUTRITION, MEMBRANE FLUIDITY AND SALIVARY ENZYMES

脂质营养、膜流动性和唾液酶

• 批准号: 3219691
• 负责人: SYED Q ALAM
• 金额: $ 14.8万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219691
• 关键词: G protein; adenosinetriphosphatase; adenylate cyclase; adrenergic receptor; biological fluid transport; cell membrane; cholesterol; dietary lipid; enzyme induction /repression; essential fatty acids; fluoridation; forskolin; guanine nucleotide binding protein; ion transport; laboratory rat; linoleate; membrane lipids; membrane proteins; nutrition related tag; ouabain; salivary glands; sodium potassium exchanging ATPase; submandibular gland; unsaturated fats

We have been studying the basic biochemical mechanisms whereby the type of dietary lipids can affect the salivary gland cell function. The working hypothesis being investigated is that dietary lipids can induce changes in structural lipids and fluidity of the cell membranes, which results in modification of membrane function. In vivo changes can be induced in membrane lipids by essential fatty acid deficiency, cholesterol-rich diets and diets containing high levels of omega-3 fatty acids. Changes in membrane lipids result in altering beta-receptor adenylate cyclase system, which consists of the receptor, G-proteins and adenylate cyclase. Further studies will be conducted using these nutritional models to determine their effects on each of the 3 components of the adenylate cyclase system in the rat submandibular salivary gland (SMSG). Studies will also be conducted with dispersed SMSG cells from rats fed various diets in order to determine if the diet-induced changes in membrane lipids and adenylate cyclase system would result in. functional changes such as the secretion of glycoproteins. Diet-induced changes in membrane fluidity will be assessed by measuring fluorescence polarization and lifetimes of the excited states for diphenylhexatriene and 16-anthroyloxy palmitate after their incorporation into the SMSG membranes. Because of the microsomal fatty acid desaturases are considered to be important in the regulation of membrane fluidity and in the metabolism of essential fatty acids, their specific activities in the SMSG, along with their dietary modulation, will be studied.

我们一直在研究该类型的基本生化机制 饮食中的脂质可以影响唾液腺细胞的功能。 工作中 正在研究的假设是膳食脂质可以引起 结构脂质和细胞膜的流动性，这导致 膜功能的改变。 体内的变化可以被诱导 必需脂肪酸缺乏、富含胆固醇的饮食导致膜脂 以及含有高水平 omega-3 脂肪酸的饮食。 变化 膜脂导致改变β受体腺苷酸环化酶系统， 它由受体、G 蛋白和腺苷酸环化酶组成。 更远 将使用这些营养模型进行研究以确定其 对腺苷酸环化酶系统的三个组成部分中的每一个的影响 大鼠颌下唾液腺（SMSG）。 还将进行研究 用来自喂食不同饮食的大鼠的分散的 SMSG 细胞来确定 如果饮食引起膜脂和腺苷酸环化酶系统的变化 将导致功能变化，例如糖蛋白的分泌。 饮食引起的膜流动性变化将通过测量来评估 荧光偏振和激发态寿命 二苯基己三烯和 16-蒽酰氧基棕榈酸酯合并后 进入 SMSG 膜。 由于微粒体脂肪酸去饱和酶 被认为对膜流动性的调节很重要 在必需脂肪酸的代谢中，它们的特定活性 SMSG 及其饮食调节将被研究。