TEMPOROMANDIBULAR JOINT--CELLULAR AND MOLECULAR BIOLOGY

颞下颌关节——细胞和分子生物学

• 批准号: 3462352
• 负责人: REGINA LANDESBERG
• 金额: $ 10.78万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3462352
• 关键词: animal tissue; articular cartilage; autocrine; cell differentiation; cell growth regulation; cell sorting; chondrocytes; collagen; fibroblast growth factor; fibronectins; growth factor; high performance liquid chromatography; insulinlike growth factor; interleukin 1; mandibular condyle; messenger RNA; molecular biology; osteocalcin; paracrine; phenotype; platelet derived growth factor; prostaglandin E; protein biosynthesis; protein purification; protein structure function; proteoglycan; recombinant DNA; temporomandibular joint; tissue /cell culture; transforming growth factors

Knowledge about the structure, function and cellular makeup of the temporomandibular joint (TMJ) is critical in order to understand both the normal and pathological biology of this joint. Additionally, the role of the mandibular condyle in growth and development of the craniofacial complex must be elucidated in order to better treat dental malocclusions and other facial deformities. For these reasons we have embarked on a cellular and molecular study of the tissues comprising the TMJ. The goals of this proposal are to 1) isolate and characterize cells from the TMJ meniscus, the condylar articular surface and the condylar growth center, 2) devise a separation technique for the partitioning of these cells into specific phenotypic sub-populations, 3) define the effects of growth factors on the modulation of the cell phenotypes and 4) test the hypothesis that some of the cells in the TMJ are autocrine regulatory cells. This work has clinical significance because of the fact that temporomandibular joint disorders are the most common facial pain condition seen by medical and dental providers. These joint disorders represent a cluster of diseases of the masticatory apparatus which range from neuromuscular to rheumatoid disorders. Knowledge of the natural history, course, and causative agents of temporomandibular joint disorders is limited. Previous investigations into the cellular, biochemical, and molecular structure of the tissues comprising the TMJ are few in number and have failed to address the regulatory and reparative processes which occur within this joint. This proposal will attempt to delineate some of the biochemical and developmental processes in the TMJ by using sophisticated cell separation, biochemical and molecular biological techniques. The cell separation techniques will utilize countercurrent centrifugal elutriation, whereby cells can be separated and collected on the basis of size. Biochemical parameters which will be studied include all aspects of matrix synthesis, cell proliferation and differentiation and their regulation by known regulatory factors. Molecular techniques will be used to identify points of regulation (i.e. transcriptional vs. translational) for the regulatory factors. Finally, using both recombinant DNA technology and newly developed assays for the growth factors, transforming growth factor beta1 and basic fibroblast growth factor, we will determine if cells from the TMJ meniscus and mandibular condyle participate in paracrine and/or autocrine regulatory pathways.

有关结构，功能和细胞构成的知识 颞下颌关节（TMJ）至关重要，以了解 该关节的正常和病理生物学。 另外， 颅面生长和发育中的下颌con 必须阐明复合物，以更好地治疗牙齿层壳 和其他面部畸形。 由于这些原因，我们开始了 包含TMJ的组织的细胞和分子研究。 该提案的目标是1）隔离并表征了细胞 TMJ半月板，dyoltar关节表面和condolar生长 中心，2）设计一种分隔技术 细胞成特定的表型亚种群，3）定义 生长因子调节细胞表型，4）测试 假设TMJ中的某些细胞是自分泌调节的假设 细胞。 这项工作具有临床意义，因为 颞下颌关节疾病是最常见的面部疼痛疾病 由医疗和牙科提供者看到。 这些关节疾病代表 咀嚼器疾病簇，范围从 神经肌肉至类风湿病。 了解自然历史， 颞下颌关节疾病的病程和病因是 有限的。 先前研究了细胞，生化和 包含TMJ的组织的分子结构数量很少，并且 未能解决发生的监管和修复过程 在这个关节内。 该提议将尝试描述一些 TMJ中的生化和发展过程通过使用复杂的 细胞分离，生化和分子生物学技术。 细胞分离技术将利用反电流离心机 放逐，可以根据细胞分离和收集细胞 尺寸。 将研究的生化参数包括 基质合成，细胞增殖和分化及其 通过已知的调节因素进行调节。 将使用分子技术 确定调节点（即转录与翻译） 对于监管因素。 最后，使用两种重组DNA技术 并新开发了增长因素的测定法，改变了增长 因子β1和基本成纤维细胞生长因子，我们将确定细胞是否是否 来自TMJ弯面条和下颌con脚旁分泌 和/或自分泌调节途径。