LONG-TERM MAINTENANCE OF PULMONARY TYPE II CELLS

II 型肺细胞的长期维持

• 批准号: 3448872
• 负责人: NEAL R METTLER
• 金额: $ 5.76万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3448872
• 关键词: NADPH cytochrome c2 reductase; biotechnology; cell differentiation; cytochrome P450; cytoskeleton; density gradient ultracentrifugation; elastin; electron microscopy; enzyme mechanism; extracellular matrix; fibronectins; glucose metabolism; growth media; laboratory rabbit; laboratory rat; laminin; lipid metabolism; microsomes; mixed tissue /cell culture; morphology; neurotrophic factors; organ culture; oxygen consumption; phospholipids; protein biosynthesis; pulmonary surfactants; tissue /cell culture; unspecific monooxygenase

This project is designed to develop a culture medium and environment which will enable type II cells to maintain differentiated functions in long term primary culture. The two functions to be studied are synthesis of surfactant phospholipids at levels approaching those for freshly isolated and cultured cells and the maintenance of enzyme activity associated with the microsomal mixed function oxidase system. The project is divided into 2 parts. The first will evaluate the isolation method for type II cells including use of proteolytic enzyme, method of lung exposure to it and supplementation of isolation medium. This will allow isolation of cells in the best possible condition. Using type II cells isolated according to these results, a culture medium will be developed containing hormones and other factors which will help maintain the differentiated properties listed above. The second portion of this project will evaluate type II cells on a extracellular matrix or its components. These will include fibronectin, laminin and a cellular lung sponge. The development of a long term primary culture of type II cells with retention of differentiated functions will remove a major restriction to the use of this cell in the study of pulmonary biology.

该项目旨在开发一种文化媒介和环境 将使II型细胞长期保持分化功能 主流文化。 要研究的两个函数是综合 表面活性剂磷脂的水平接近新鲜分离的水平 和培养细胞以及与相关酶活性的维持 微粒体混合功能氧化酶系统。 该项目分为 2 部分。 第一个将评估 II 型细胞的分离方法 包括蛋白水解酶的使用、肺部暴露于该酶的方法以及 补充隔离介质。 这将允许分离细胞 尽可能最好的状态。 使用根据以下方法分离的 II 型细胞 根据这些结果，将开发出一种含有激素和 其他有助于维持列出的差异化属性的因素 多于。 该项目的第二部分将评估 II 型细胞 细胞外基质或其成分。 这些将包括纤连蛋白， 层粘连蛋白和细胞肺海绵。 II型细胞长期原代培养物的开发 保留差异化功能将消除主要限制 该细胞在肺部生物学研究中的应用。