A NEW INTRARENAL NEUROVASCULAR STRUCTURE

一种新的肾内神经血管结构

• 批准号: 3448939
• 负责人: DAVID S KNIGHT
• 金额: $ 3.97万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3448939
• 关键词: 6 hydroxydopamine; afferent nerve; arterioles; axon; catecholamines; chemoreceptors; dopamine beta monooxygenase; electron microscopy; fluorescence microscopy; histochemistry /cytochemistry; immunochemistry; mechanoreceptors; microscopy; neurotransmitters; renal artery; spinal nerves; sympathetic nervous system; synaptic vesicles; tyrosine 3 monooxygenase

General studies of intrarenal catecholamine-containing nerves have shown that these sympathetic nerves form dense plexuses around the renal arteries and veins. Intrarenal sensory nerves are both chemoreceptive and mechanoreceptive, but data on receptor distribution are minimal and the fine structure of sensory terminals and the neurotransmitters used by these nerves are unknown. The investigator has examined with the electron microscope, a new intrarenal structure consisting of a cluster of axonal enlargements that partially surround arterioles and contain catecholamine in high concentration. Functionally, axons of this neurovascular structure may be efferent or afferent. The objectives of the proposed project are to study the anatomical relationships between intrarenal axonal enlargements and the renal arterial tree. Localization of sensory and autonomic nerve cell bodies innervating the kidneys and systematic section of their axons will help to establish the sensory or autonomic nature of the neurovascular structure. Immunohistochemical localization of TH, DBH, substance P, somatostatin and VIP will help to characterize the neurotransmitter used by these nerves. These data will be useful in the process of determining whether the modified axons and associated arterioles contribute significantly to renal function in healthy or in hypertensive animals.

对肾内含儿茶酚胺神经的一般研究表明 这些交感神经在肾动脉周围形成密集的神经丛 和静脉。 肾内感觉神经既具有化学感受性又具有化学感受性 机械感受，但受体分布的数据很少，并且 感觉末梢的精细结构及其使用的神经递质 神经未知。 研究人员用电子检查 显微镜下，一种新的肾内结构，由轴突簇组成 部分围绕小动脉并含有儿茶酚胺的肿大 在高浓度下。 从功能上讲，这种神经血管结构的轴突 可能是传出的或传入的。 拟议项目的目标是 研究肾内轴突增大之间的解剖关系 和肾动脉树。 感觉和自主神经的定位 支配肾脏的细胞体及其轴突的系统部分 将有助于建立神经血管的感觉或自主神经性质 结构。 TH、DBH、P 物质的免疫组织化学定位 生长抑素和 VIP 将有助于表征神经递质 这些神经。 这些数据将在确定过程中有用 修饰的轴突和相关的小动脉是否有助于 对健康或高血压动物的肾功能有显着影响。