MYCOBACTERIUM LEPRAE TAXONOMY AND GENETICS

麻风分枝杆菌分类学和遗传学

• 批准号: 3565491
• 负责人: JOSEPHINE E CLARK-CURTISS
• 金额: $ 11.29万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3565491
• 关键词: Cercopithecidae; Edentata; Mycobacterium leprae; Pan; communicable disease diagnosis; connective tissue disorder; diagnosis quality /standard; disease /disorder model; endonuclease; genetic library; genetic markers; genome; laboratory rabbit; microorganism genetics; microorganism immunology; molecular cloning; molecular genetics; nervous system disorder; nucleic acid hybridization; restriction endonucleases; restriction mapping; systematic biology; vaccines

Studies are proposed that will lead to an understanding of the genes and gene products of Mycobacterium leprae that are important in the pathogenicity of this organism through molecular and functional analyses of recombinant molecules from genomic libraries. Detailed taxonomic and strain diversity studies will be undertaken initially as well as studies to provide information concerning the existence of an animal reservoir. This information is essential for developing means for accurate diagnosis and prevention (by Immunization) of leprosy. Specifically, we will: (1) employ DNA restriction fragment length polymorphism analyses to (a) assess relatedness among the genomes of M. leprae infecting humans, cultivable acid-fast bacteria obtained from leprosy patients, non-cultivable mycobacteria obtained from animals (armadillos, Mangabey monkeys, chimpanzees) with leprosy-like disease and cultivable mycobacteria isolated from soil and (b) assess relatedness or divergence in the genomes of M. leprae strains isolated from leprosy patients in different parts of the world. (2) identify and analyze genes specifying enzymes that might contribute to the ability of M. leprae to destroy connective tissue and/or to attach to, invade and multiply in nerves. The research will be done in conformance with the NIH Guidelines for Recombinant DNA Research. The studies will make use of the technologies of molecular genetics bacterial genetics, microbiology, immunology and biochemistry.

提出了研究，这将导致对 麻风分枝杆菌的基因和基因产物 通过分子的病原体重要 基因组重组分子的功能分析 库。 详细的分类学和应变多样性研究将是 最初进行的研究以及提供信息 关于动物水库的存在。 此信息 对于制定精确诊断和 麻风病的预防（通过免疫）。 具体来说，我们将： （1）采用DNA限制片段长度多态性 分析（a）评估Leprae基因组之间的相关性 感染人类，可从 麻风病患者，不可培养的分枝杆菌 动物（Armadillos，Mangabey猴子，黑猩猩）与 从中分离出麻风病和可栽培的分枝杆菌 土壤和（b）评估M的相关性或差异。 从麻风病患者中分离出的麻风病毒菌株 世界。 （2）识别和分析指定酶的基因 可能有助于M. Leprae销毁结缔组织的能力 组织和/或附着在神经中，侵入和繁殖。 这 研究将符合NIH指南 重组DNA研究。 研究将利用 分子遗传学的技术细菌遗传学， 微生物学，免疫学和生物化学。