HAIR FOLLICLE KERATIN GENE EXPRESSION

毛囊角蛋白基因表达

• 批准号: 3446335
• 负责人: ARTHUR P BERTOLINO
• 金额: $ 5.21万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446335
• 关键词: cell differentiation; complementary DNA; endonuclease; gene expression; genetic translation; growth /development; hair follicle; hormone regulation /control mechanism; hybrid cells; in situ hybridization; keratin; laboratory mouse; laboratory rabbit; messenger RNA; molecular biology; molecular cloning; nucleic acid sequence; restriction mapping

The goal of this project is to define methodologies by which to investigate the mechanisms of hair follicle growth and differentiation at the molecular level. The initial approach will focus upon follicle-specific protein gene expression in the mouse. This model system is uniquely advantageous as it offers a wide variety of relevant mutants of defined genetic background. The current project will involve preparation of molecular probes which, in subsequent projects, will be used to examine follicular regulatory mechanisms. Recombinant DNA technology will be used to prepare cloned complementary DNA probes with specificities for major hair keratin mRNAs. These probes will be characterized by positive hybridization selection translation assays to determine individual keratin specificities and appropriate specific fragments will be subcloned. Further characterization will be achieved by restriction mapping and nucleotide sequence analyses. Optimal conditions for in situ hybridization analyses using these probes directly in tissue sections will be determined. In this way, a technique will be developed by which to assess qualitative and semi-quantitative distributions of hair follicle keratin mRNAs. Through these studies a foundation will be established upon which the longer-term objective of understanding corresponding pathological processes of hair follicles may be reached.

该项目的目的是定义研究方法 分子的毛囊生长和分化的机制 等级。 初始方法将集中于卵泡特异性蛋白质基因 在鼠标中的表达。 这个模型系统具有独特的优势 提供定义的遗传背景的各种相关突变体。 当前项目将涉及分子探针的制备，该探针在 随后的项目将用于检查卵泡调节性 机制。 重组DNA技术将用于制备克隆的互补DNA 具有特异性的探针。 这些探针会 以阳性杂交选择翻译分析为特征 确定个体角蛋白特异性和适当的特定性 碎片将被亚克隆。 进一步的表征将通过 限制映射和核苷酸序列分析。 最佳条件 直接在组织中使用这些探针进行原位杂交分析 将确定部分。 这样，将开发一种技术 评估头发的定性和半定量分布 卵泡角蛋白mRNA。 通过这些研究，将建立一个基础 理解相应病理过程的长期目标 可以达到毛囊的毛囊。

项目成果

Chromosomal localization of mouse hair keratin genes.

小鼠毛发角蛋白基因的染色体定位。

• DOI: 10.1111/j.1749-6632.1991.tb24378.x
• 发表时间: 1991
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: Compton,JG;Ferrara,DM;Yu,DW;Recca,V;Freedberg,IM;Bertolino,AP
• 通讯作者: Bertolino,AP

Cloning and characterization of a mouse type I hair keratin cDNA.

小鼠 I 型毛发角蛋白 cDNA 的克隆和表征。

• DOI: 10.1111/1523-1747.ep12476901
• 发表时间: 1988
• 期刊: The Journal of investigative dermatology
• 作者: Bertolino,AP;Checkla,DM;Notterman,R;Sklaver,I;Schiff,TA;Freedberg,IM;DiDona,GJ
• 通讯作者: DiDona,GJ

Differential expression of type I hair keratins.

I 型头发角蛋白的差异表达。

• DOI: 10.1111/1523-1747.ep12874436
• 发表时间: 1990
• 期刊: The Journal of investigative dermatology
• 作者: Bertolino,AP;Checkla,DM;Heitner,S;Freedberg,IM;Yu,DW
• 通讯作者: Yu,DW

Transient expression of mouse hair keratins in transfected HeLa cells: interactions between "hard" and "soft" keratins.

转染的 HeLa 细胞中小鼠毛发角蛋白的瞬时表达：“硬”角蛋白和“软”角蛋白之间的相互作用。

• DOI: 10.1111/1523-1747.ep12480706
• 发表时间: 1991
• 期刊: The Journal of investigative dermatology
• 作者: Yu,DW;Pang,SY;Checkla,DM;Freedberg,IM;Sun,TT;Bertolino,AP
• 通讯作者: Bertolino,AP