NON-RESPONDERS FOLLOWING VENOUS OCCLUSION

静脉闭塞后无反应者

基本信息

批准号：
3440183
负责人：
KAROL A LACROIX
金额：
$ 10.33万
依托单位：
UNIVERSITY OF NEW HAMPSHIRE
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-04-01 至 1994-03-31
项目状态：
已结题

项目摘要

DESCRIPTION: (Adapted from the investigator's abstract). The purpose of this project is to: (1) identify a population of non-responders and (2) to determine if there is any abnormality which can be detected with selected clinical assays in the activation or inhibition of fibrinolysis within this group. Non-responders are individuals who have normal fibrinolytic activity at rest but do not demonstrate the expected in vitro increase following the application of stressors such as venous occlusion or acute exercise. In this proposed study, fasting venous blood samples will be drawn pre- and post-venous occlusion from 200 volunteers between the ages of 18 and 25 years and screened for fibrinolytic activity using the fibrin plate assay. Those subjects who demonstrate an increased fibrinolysis following venous occlusion will be termed responders and those who do not will be classified as non-responders. Plasma from both groups will be analyzed for factors associated with the activation or inhibition of fibrinolysis including free and total tissue plasminogen activator (t-PA), PAI-1 which is the major inhibitor to t-PA, Protein C antigen and activity levels, kallikrein, Factor XII, plasminogen, alpha-2 antiplasmin, serum lipids, lipoproteins, and apolipoproteins. Thrombosis occurs when there is an imbalance in the hemostatic mechanism. It is associated with excessive or uncontrolled clotting, decreased levels of naturally occurring inhibitors such as antithrombin III and Protein C, or hypofibrinolysis. This study will determine if healthy non-responders have a detectable abnormality associated with the activation or inhibition of fibrinolysis following the application of a stressor, and may represent a means to screen individuals who could be at risk for thrombotic. It also provides the impetus for future studies on family members of nonresponders to determine if they have the same diminished fibrinolytic response following venous occlusion or have experienced thrombotic complications. Lastly, this study will provide valuable specimens for additional hemostasis determinations in other laboratories.

描述：（根据研究者的摘要进行了改编）。目的该项目是：（1）确定非反应人群，（2）确定是否有任何异常可以通过选定检测到在此激活或抑制纤维蛋白溶解的临床测定团体。非反应者是患有正常纤维蛋白水解的个体休息时的活动，但不能证明预期的体外增加遵循静脉阻塞或急性等应激源锻炼。在这项拟议的研究中，禁食静脉血液样本将是从年龄段的200名志愿者中抽取前后的闭塞 18年和25年，并使用纤维蛋白进行纤维蛋白水解活性板测定。那些表现出增加纤维蛋白溶解的受试者静脉阻塞后将被称为响应者，而那些不响应者将被归类为非反应者。两组的等离子体将是分析与激活或抑制相关的因素纤维蛋白溶解包括游离和总组织纤溶酶原激活剂（T-PA）， PAI-1是T-PA，蛋白C抗原和活性的主要抑制剂水平，kallikrein，因子XII，纤溶酶原，α-2抗血流蛋白，血清脂质，脂蛋白和载脂蛋白。当存在时，血栓形成发生止血机制的不平衡。它与过度有关或不受控制的凝结，自然发生的水平降低抑制剂，例如抗凝血酶III和蛋白C或低纤维蛋白溶解。这项研究将确定健康的非反应器是否具有可检测到的与纤维蛋白溶解的激活或抑制有关的异常遵循压力源的应用，并且可能代表筛选可能有血栓形成风险的人。它也提供未来对非反应者家庭成员的未来研究的动力确定它们是否具有相同的纤维蛋白水解响应之后静脉阻塞或经历了血栓形成并发症。最后，这项研究将为额外止血提供宝贵的标本其他实验室的决定。