BACTERIAL BINDING MOLECULES ON THE MACROPHAGE SURFACE

巨噬细胞表面的细菌结合分子

• 批准号: 3436714
• 负责人: THOMAS G PISTOLE
• 金额: $ 10.4万
• 依托单位: UNIVERSITY OF NEW HAMPSHIRE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3436714
• 关键词: Salmonella typhimurium; Staphylococcus epidermidis; Streptococcus agalactiae; affinity chromatography; antigen receptors; antireceptor antibody; binding proteins; biochemical evolution; chemical binding; laboratory mouse; macrophage; membrane activity; peptidases; protein purification; protein structure; tissue /cell culture

The overall goal of this project is to gain a better understanding of one aspect our innate antimicrobic defenses, namely that involving the macrophage. While the contributions to mammalian defense by antibody and lymphocytes are well-recognized, the activity of our constitutive defense, in the absence of antigen- directed events, is less understood. The antibacterial effects of macrophages are particularly significant in individuals manifesting inadequate specific immunity due to age (e.g., neonates), genetics (e.g., inherited immunodeficiency), or infection (e.g., acquired immunodeficiency,). Our approach will be to examine the ability of macrophages to recognize selected pathogenic bacterial in in vitro binding assays. These studies will provide information on microbial recognition by macrophages as well as a monitoring system for subsequent studies. The second part of this study involves a characterization of the lectin-like receptor on the intact macrophage through use of proteases and cellular modulating agents and by blocking studies with various glycoconjugates. The third specific goal of this study is to purify the bacterial binding receptor from the macrophage membrane and assess its ability to bind to bacteria. Antibody to the receptor will be tested for its ability to block bacterial binding. A long-term objective of this study is to determine whether bacterial-binding receptors on mammalian macrophages represent an evolutionarily conserved defense mechanism.

该项目的总体目标是获得更好的理解 一方面是我们与生俱来的抗菌防御能力，即 涉及巨噬细胞。 虽然对哺乳动物的贡献 抗体和淋巴细胞的防御已得到广泛认可， 在没有抗原的情况下，我们的组成性防御活动 定向事件，了解较少。 的抗菌作用 巨噬细胞在个体中尤其重要 由于年龄（例如新生儿）、遗传而导致的特异性免疫力不足 （例如遗传性免疫缺陷）或感染（例如获得性免疫缺陷） 免疫缺陷，）。 我们的方法是检查能力 巨噬细胞识别选定的致病细菌 体外结合测定。 这些研究将提供以下信息： 巨噬细胞的微生物识别以及监测系统 以供后续研究。 本研究的第二部分涉及 完整凝集素样受体的表征 通过使用蛋白酶和细胞调节剂的巨噬细胞 以及通过各种糖复合物的阻断研究。 第三个 这项研究的具体目标是纯化细菌结合 巨噬细胞膜受体并评估其能力 与细菌结合。 将测试该受体的抗体 阻止细菌结合的能力。 此举的长期目标 研究的目的是确定细菌结合受体是否 哺乳动物巨噬细胞代表进化上保守的防御 机制。

项目成果

A quantitative method for measuring the adherence of group B streptococci to murine peritoneal exudate macrophages.

一种测量 B 族链球菌与小鼠腹腔渗出物巨噬细胞粘附的定量方法。

• DOI: 10.1016/0022-1759(92)90195-y
• 发表时间: 1992
• 期刊: Journal of immunological methods
• 影响因子: 2.2
• 作者: Sloan,AR;Pistole,TG
• 通讯作者: Pistole,TG

Characterization of the murine macrophage receptor for group B streptococci.

B 族链球菌的鼠巨噬细胞受体的表征。

• DOI: 10.1016/s0934-8840(11)80825-x
• 发表时间: 1993
• 期刊: Zentralblatt fur Bakteriologie : international journal of medical microbiology
• 作者: Sloan,AR;Pistole,TG
• 通讯作者: Pistole,TG