REGULATION OF ANDROGEN RECEPTOR ACTIVITY

雄激素受体活性的调节

• 批准号: 3426189
• 负责人: VIRENDRA B MAHESH
• 金额: $ 3.12万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-15 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3426189
• 关键词: DNA footprinting; androgen inhibitor; androgen receptor; androgens; gel mobility shift assay; genetic enhancer element; genetic promoter element; genetic transcription; hormone regulation /control mechanism; laboratory rat; messenger RNA; nuclear runoff assay; prostate; prostate neoplasms; receptor binding; tissue /cell culture; transfection

The major objectives of this proposal are to obtain training and experience in molecular biology techniques so that they can be used in the Principal Investigator's laboratory in his research. The training will be provided by Drs. Frank French and Elizabeth Wilson at the University of North Carolina at Chapel Hill. The designated trainee will be Jaime Steinsapir, M.D., Ph.D. who will work for at least one year after completion of training in the P.I.'s laboratory. The research to be conducted is as follows: Prostate cells are responsive to androgens by virtue of the presence of functional androgen receptors (AR). The goal of these studies is to determine mechanisms by which androgens and antiandrogens control AR activity and turnover in normal and neoplastic prostate cells. The effects of androgen withdrawal on androgen and antiandrogen control of mRNA-AR levels and initial transcription rate of the AR gene will be examined in rat ventral prostate and human prostate cancer cells (LNCaP). The assessment of the effects of androgen and antiandrogens on the transcriptional activity of the AR gene upstream enhancer/promoter coupled with studies on AR binding to promoter sequences should determine specific response elements involved in AR autoregulation of the AR gene. The results will further help to understand the molecular mechanisms of regulation of AR gene transcription by androgens and antiandrogens. This project will be used as a basis for training in the use of molecular biology techniques in hormone receptor synthesis and turnover.

该提案的主要目标是获得培训和经验 在分子生物学技术中，可以将其用于主 调查员的研究中的实验室。 将提供培训 由Drs。北部大学的弗兰克·法国和伊丽莎白·威尔逊 卡罗来纳州教堂山。 指定的实习生将是Jaime Steinsapir， 医学博士谁将在完成后至少工作一年 在P.I.的实验室进行培训。 进行的研究是 以下内容： 前列腺细胞凭借存在对雄激素的反应 功能性雄激素受体（AR）。 这些研究的目的是 确定雄激素和抗雄激素控制AR的机制 正常和肿瘤前列腺细胞的活性和周转。 效果 mRNA-AR的雄激素和抗雄激素控制的雄激素戒断 AR基因的水平和初始转录率将在 大鼠腹前列腺和人类前列腺癌细胞（LNCAP）。 评估雄激素和抗雄激素对 AR基因上游增强子/启动子的转录活性耦合 通过对AR与启动子序列结合的研究应确定特定 AR基因自动调节涉及的响应元件。 这 结果将进一步帮助了解分子机制 通过雄激素和抗雄激素调节AR基因转录。 该项目将用作培训分子的培训的基础 激素受体合成和周转率中的生物学技术。