HIV-1 AFFECTS MONONUCLEAR PHAGOCYTE ANTIFUNGAL ACTIVITY

HIV-1 影响单核吞噬细胞抗真菌活性

• 批准号: 3422640
• 负责人: MIRIAM L CAMERON
• 金额: $ 2.69万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3422640
• 关键词: AIDS; Cryptococcus neoformans; RNA directed DNA polymerase; antiAIDS agent; cryptococcosis; human immunodeficiency virus 1; human subject; immunofluorescence technique; immunoregulation; in situ hybridization; monocyte; nitrogen; opportunistic infections; oxidation reduction reaction; phagocytosis; surface antigens; tissue /cell culture; virus protein

DESCRIPTIONS: (Adapted from Applicant's Abstract). The aim of this proposal is to study the host response to HIV infection by examining the effect of in vivo HIV-1 infection on mononuclear phagocyte antimicrobial and immunoregulatory-immunomodulation functions. In particular, mononuclear phagocyte interactions with Crytococcus neoformans will be studied. The specific aims are: 1) Does HIV-1 infection of mononuclear phagocytes in vivo affect anticryptococcal function? Functions to be measured include phagocytosis, fungistasis, nitrogen oxidation, oxidative metabolism, cell surface antigen expression. 2) Can HIV-1 infection be demonstrated by reverse transcriptase activity, p24 antigen, immunofluorescence, and in situ hybridization in mononuclear phagocytes from HIV-1 seropositive individuals? 3) Are in vitro mononuclear phagocyte function and clinical stage of HIV-1 infection correlated? 4) Does specific in vivo anti-retroviral therapy decrease HIV-1 infection/latency in mononuclear phagocytes? 5) Does in vivo anti-retroviral therapy improve HIV-1 infected mononuclear phagocyte antimicrobial and immunomodulatory functions? The investigator believes that determing the effect of HIV-1 infection on mononuclear phagocyte-mediated anticryptococcal function will help to understand host-virus interactions, may serve as a marker for disease progression, and could be used as a model for evaluating anti-retroviral therapy.

描述：（改编自申请人的摘要）。 这个目的 提案是通过检查宿主对艾滋病毒感染的反应 体内HIV-1感染对单核吞噬细胞抗微生物的影响 和免疫调节 - 免疫调节功能。 尤其， 单核吞噬细胞与Crytococcus Neoformans的相互作用将是 研究。 具体目的是：1）单核的HIV-1感染是否会感染 体内的吞噬细胞会影响抗癌功能？ 功能 测得的包括吞噬作用，局势，氮氧化，氧化 代谢，细胞表面抗原表达。 2）HIV-1感染可以 通过逆转录酶活性P24抗原证明 免疫荧光和单核吞噬细胞的原位杂交 来自HIV-1血清阳性个体？ 3）是体外单核吞噬细胞 HIV-1感染的功能和临床阶段相关？ 4）做 特异性体内抗逆转录病毒疗法降低了HIV-1感染/潜伏期 在单核吞噬细胞中？ 5）在体内抗返回病毒疗法会改善 HIV-1感染的单核吞噬细胞抗菌和免疫调节性 功能？ 调查人员认为确定HIV-1的影响 单核吞噬细胞介导的抗癌功能的感染将 帮助了解主机病毒互动，可以作为标记 疾病进展，可以用作评估的模型 抗逆转录病毒疗法。