EFFECT OF MARIJUANA ON EXPERIMENTAL SYPHILIS IN RABBITS

大麻对兔子实验性梅毒的影响

• 批准号: 3424212
• 负责人: LOIS J PARADISE
• 金额: $ 6.55万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3424212
• 关键词: Treponema pallidum; active immunization; antibacterial antibody; biopsy; cannabinoids; disease /disorder model; disease /disorder proneness /risk; dosage; drug adverse effect; enzyme linked immunosorbent assay; hemagglutination test; histology; host organism interaction; immunity; immunofluorescence technique; immunologic assay /test; immunosuppression; laboratory rabbit; marijuana abuse; microscopy; syphilis; syphilis test

The long-term objective of the proposed investigation is to determine the effect of 'marijuana usage on syphilis. Since studies of the immunogenic components of the syphilitic treponeme have been progressing rapidly in the past few years, the potential for development of a protective vaccine with which populations at risk of exposure to other persons with syphilis could be immunized is rising. Currently, one of the groups in which the incidence of syphilis is increasing consists of drug abusers. There is more and more evidence that marijuana components are suppressive for the immune response. It is important to determine, therefore, whether or not the immunosuppressive activity of marijuana will prevent users from being able to be immunized successfully by syphilis vaccines. The specfic aims of this project are (1) to measure in the standard rabbit model for human syphilis whether or not delta-9-tetrahydrocannabinol (THC) the active component in marijuana, enhances infectivity of the syphilitic treponemes and (2) to measure whether or not on-going treatment with THC inhibits the induction of protective immunity to syphilis by vaccination. Two-way dose-response experiments will test a range of concentrations of THC against a range of infecting doses of Treponema pallidum subsp. pallidum, the causative agent of syphilis. Optimal effective doses of THC for numbers of infecting organisms will be calculated. For investigation of the effect of THC on immune response to syphilis, the dose-related effect of the drug on active immunization by administration of a vaccine and by previous infection will be determined. The level of protective immunity of rabbits in both cases will be challenged by inoculation of graded doses of treponemes at selected times after THC-treatment is initiated. Results of these experiments will demonstrate whether THC does have an inhibitory effect on host resistance mechanisms to syphilis and the need for additional investigation of the mechanism of action of THC involved.

拟议调查的长期目标是确定 使用大麻对梅毒的影响。 自从研究了 梅毒螺旋体的免疫原性成分一直在进展 近年来发展迅速，潜力巨大 有接触其他风险的人群可接种保护性疫苗 可以接种疫苗的梅毒患者正在增加。 目前，其中之一 梅毒发病率上升的群体包括 吸毒者。 越来越多的证据表明大麻成分 对免疫反应有抑制作用。 重要的是要确定， 因此，大麻是否具有免疫抑制活性 将阻止用户成功免疫 梅毒疫苗。 该项目的具体目标是（1） 在人类梅毒的标准兔子模型中是否 δ-9-四氢大麻酚（THC）是大麻中的活性成分， 增强梅毒密螺旋体的传染性，并且 (2) 测量 持续使用 THC 治疗是否会抑制诱导 通过疫苗接种获得对梅毒的保护性免疫力。 双向剂量反应 实验将测试一系列 THC 浓度与一系列 梅毒螺旋体亚种的感染剂量。苍白球，致病因素 梅毒的媒介。 THC 的最佳有效剂量 将计算感染生物体。 为了调查效果 THC 对梅毒免疫反应的影响与剂量相关 通过注射疫苗和通过主动免疫的药物 将确定以前的感染情况。 保护性免疫力水平 在这两种情况下，兔子都会受到分级接种的挑战 THC 治疗开始后，在选定的时间给予密螺旋体剂量。 这些实验的结果将证明 THC 是否确实具有 对宿主梅毒耐药机制的抑制作用及需求 对所涉及的 THC 作用机制进行进一步研究。