ENCYCLOPEDIA OF E COLI GENES AND METABOLISM

大肠杆菌基因和代谢百科全书

• 批准号: 3421782
• 负责人: PETER D KARP
• 金额: $ 18.87万
• 依托单位: SRI INTERNATIONAL
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1995-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3421782
• 关键词: Escherichia coli; alternatives to animals in research; bacterial genetics; chemical reaction; chromosomes; computer human interaction; computer system design /evaluation; data collection; enzyme activity; enzyme inhibitors; genetic mapping; information retrieval; information systems; microorganism metabolism; molecular genetics; molecular weight; nucleic acid sequence; protein sequence

SRI International (SRI) proposes to collect data on the genes and metabolism of Escherichia coli, and to encode these data in a computer database that is accessible to all scientists. We will compile data information on intermediary metabolism from the biological literature. A collaborator will provide us with a database on the genes of E coli. We will encode the genetic and metabolic information in database form. In addition, SRI will implement a graphical user interface to the metabolic data, and integrate the metabolic graphical interface with a graphical user interface for genetic data developed at the Jackson Laboratory. The resulting program will allow scientist to access genetic and metabolic data in a graphical, user-friendly manner. In addition, we will distribute the database (to be called EcoCyc) in several different database formats so that scientists can access the data in other convenient forms. The EcoCyc database will describe every reaction of the intermediary metabolism of E coli, listing the reaction equation, its EC number, cofactors, activators, and inhibitors. EcoCyc will also describe the enzymes that catalyze these reactions, listing their molecular weight, subunit composition, encoding genes, and cross reference to protein and nucleic-acid sequence databases. For each gene EcoCyc will list the gene name, and the starting and ending position of the gene within the E coli chromosome. Literature citations will be included in all the preceding items. EcoCyc will be valuable as a reference source for many scientists. Molecular biologists and biotechnologists designing a cloning project will have instant access to the best current information on map locations, identity of linked genes and their functions, lists of available clones, and locations of pertinent restriction sites. Microbial geneticists who work with bacteria other than E coli may find facile access to E coli data useful as a point of reference for gene functions, as well as for similarities and differences of gene-enzyme relationships in other organisms. Molecular evolutionists interested in ancestral relationships among genes, enzymes and pathways could use the database to search for examples of duplication and divergence. EcoCyc will also be useful to scientists who perform quantitative simulations of metabolic pathways, and to those who design variants of biochemical pathways for industrial applications.

SRI International (SRI) 提议收集有关基因和 大肠杆菌的代谢，并将这些数据编码到计算机中 所有科学家都可以访问的数据库。 我们将整理数据 来自生物学文献的中间代谢信息。 合作者将为我们提供有关大肠杆菌基因的数据库。 我们将以数据库的形式对遗传和代谢信息进行编码。 此外，SRI 将实施图形用户界面 代谢数据，并将代谢图形界面与 杰克逊开发的遗传数据图形用户界面 实验室。 由此产生的程序将允许科学家访问 以图形化、用户友好的方式获取遗传和代谢数据。 在 此外，我们将在以下位置分发数据库（称为 EcoCyc）： 几种不同的数据库格式，以便科学家可以访问 其他方便形式的数据。 EcoCyc数据库将描述中介的每一个反应 大肠杆菌的代谢，列出反应方程式，其 EC 数， 辅助因子、激活剂和抑制剂。 EcoCyc 还将描述 催化这些反应的酶，列出它们的分子量， 亚基组成、编码基因以及蛋白质和蛋白质的交叉参考 核酸序列数据库。 对于每个基因，EcoCyc 将列出 基因名称，以及基因在基因组中的起始和结束位置 大肠杆菌染色体。 文献引用将包含在所有 前面的项目。 EcoCyc 对于许多科学家来说将是有价值的参考来源。 分子生物学家和生物技术专家设计克隆项目 将可以即时访问地图上的最新最佳信息 位置、连锁基因的身份及其功能、列表 可用的克隆以及相关限制性位点的位置。 研究大肠杆菌以外的细菌的微生物遗传学家可能会发现 轻松获取大肠杆菌数据，可作为基因参考点 功能以及基因酶的异同 其他生物体中的关系。 分子进化论者感兴趣 基因、酶和途径之间的祖先关系可以利用 数据库来搜索重复和分歧的示例。 生态循环 对于进行定量模拟的科学家来说也很有用 代谢途径的研究，以及那些设计生化途径变体的人 工业应用的途径。