AIDS VIRUS INFECTION OF HUMAN NEURONAL CELLS

艾滋病病毒感染人类神经细胞

• 批准号: 3411382
• 负责人: RUY SOEIRO
• 金额: $ 18.4万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3411382
• 关键词: AIDS; Retroviridae disease; antireceptor antibody; brain cell; cell differentiation; cell type; defective virus; electron microscopy; flow cytometry; gene expression; genetic strain; genetic transcription; genetic translation; helper T lymphocyte; human immunodeficiency virus 1; human tissue; latent virus infection; messenger RNA; molecular pathology; neoplastic cell culture for noncancer research; nervous system disorder; neuroblastoma; neurologic manifestations; neurotropic virus; transfection; ultracentrifugation; virus DNA; virus RNA; virus cytopathogenic effect; virus genetics; virus infection mechanism; virus protein; virus receptors; virus replication

AIDS virus infection has been associated with a variety of neurological syndromes ranging from peripheral neuropathy to dementia. Although the Human Immunodeficiency Virus (HIV) has been isolated from brain tissue and cerebro-spinal fluid of infected patients, evidence for wide spread nerve cell viral replication has not been demonstrated. We have preliminary evidence that restricted infection of human neuroblastoma cells occurs in tissue culture. We will test the hypothesis that restrictive (or incomplete) HIV infection occurs in cells of neuronal origin to develop a tissue culture model system for the study of HIV effects on human brain cells. These studies will test the extent of viral gene expression in a series of phenotypically different neuroblastoma cell types. We will assess whether cell dysfunction, measured in terms of alteration of specific host cell gene expression, is altered by infection with HIV. Finally, we will determine whether HIV infection affects the ability of each neuroblastoma to differentiate, and whether the level of cellular differentiation alters the degree of permissiveness of HIV infection.

艾滋病病毒感染与多种 神经综合征从周围神经病到 失智。 尽管人类免疫缺陷病毒（HIV）具有 是从脑组织和脑脊髓液中分离出来的 受感染的患者，证据表明广泛的扩散神经细胞病毒 复制尚未得到证明。 我们有初步的证据表明人类感染受到限制 神经母细胞瘤细胞发生在组织培养物中。 我们将测试 限制性（或不完全）HIV感染的假设发生在 神经元起源的细胞开发组织培养模型系统 为了研究艾滋病毒对人脑细胞的影响。 这些研究将测试A中病毒基因表达的程度 一系列表型不同的神经母细胞瘤细胞类型。 我们 将评估细胞功能障碍是否以 特定宿主细胞基因表达的改变，通过 艾滋病毒感染。 最后，我们将确定HIV感染是否影响 每个神经母细胞瘤的能力分化，以及是否 细胞分化水平改变了 艾滋病毒感染的允许性。

项目成果

Proliferative membranopathy and human immunodeficiency virus in AIDS hearts.

艾滋病心脏中的增殖性膜病和人类免疫缺陷病毒。

• 发表时间: 1989
• 期刊: Journal of acquired immune deficiency syndromes
• 作者: Flomenbaum,M;Soeiro,R;Udem,SA;Kress,Y;Factor,SM
• 通讯作者: Factor,SM